Abstract: The disclosure provides materials and methods for assessing the presence and level of polyanionic compounds in a sample that inhibit PCR reactions, including assays for the presence, and optionally the amount, of polyvinyl sulfonate, as well as materials and methods for the reduction or removal of such compounds from buffer solutions and from protein solutions using a variety of approaches, including titration-based techniques.

Abstract: This application provides a method of using aptamers to determine the presence of HCP's in a recombinant protein preparation, and provides methods of preparing a plurality of aptamers for use in the method of detecting a plurality of HCP's in a recombinant protein preparation. In accordance with the description, a method of determining the presence or absence of a plurality of HCP's in a recombinant protein preparation comprises: providing a recombinant protein preparation; providing a final pool of aptamers; combining the recombinant protein preparation with the final pool of aptamers; and determining the presence or absence of a plurality of HCP's.

Abstract: This application provides a method of using aptamers to determine the presence of HCP's in a recombinant protein preparation, and provides methods of preparing a plurality of aptamers for use in the method of detecting a plurality of HCP's in a recombinant protein preparation. In accordance with the description, a method of determining the presence or absence of a plurality of HCP's in a recombinant protein preparation comprises: providing a recombinant protein preparation; providing a final pool of aptamers; combining the recombinant protein preparation with the final pool of aptamers; and determining the presence or absence of a plurality of HCP's.