Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention is directed to methods of using novel Babesia antigen peptides in detecting Babesia spp. in a sample. The methods may be adapted to assays suitable for high blood volume screening, use for clinical diagnosis of patients with babesiosis, or assaying blood contaminated with Babesia spp., such as B. microti.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention is directed to methods of using novel Babesia antigen peptides in detecting Babesia spp. in a sample. The methods may be adapted to assays suitable for high blood volume screening, use for clinical diagnosis of patients with babesiosis, or assaying blood contaminated with Babesia spp., such as B. microti.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The present invention features, inter alia, compositions and methods useful for identifying one or more types of microorganisms, if and when present, in a sample or plurality of samples (e.g., in one or more samples tested in parallel). More specifically, the present compositions and methods can be used in, e.g., determining whether a subject has a microbial infection (e.g., a bacterial, fungal, protozoal, or viral infection), determining the identity of the microbe(s) causing the infection, and/or determining, or helping to determine, an appropriate anti-microbial treatment regimen for a subject identified as having an infection (e.g., an appropriate antibiotic, anti-fungal, anti-viral, or other treatment regimen).

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-?-D-glucan in a sample.

Abstract: The invention includes assay cassettes that can be employed during rapid flow-through binding assays. The assay cassettes can be disposable units suitable for one-time use and readily assembled to include a filter membrane carried between an upper plate and a lower plate. A pattern of channels can extend through the top plate to allow a fluid sample to be applied through the top plate and onto the filter. The bottom plate can include a plurality of channels that are aligned with the channel of the top plate and which will allow a negative pressure to be applied to the underside of the filter membrane to draw the sample through the filter. In one embodiment, the cassette includes a frangible section that allows the cassette to be divided into a first and second component.

Abstract: Systems and methods for processing membrane flow-through assays to provide more rapid testing. The systems include a plate for receiving a cartridge having a membrane which, for example, can be a membrane with antigens or antibodies fixed thereon. The agents can be pipetted onto the membrane and the systems can be activated to agitate the cartridge to evenly distribute the agents over the membrane, and a vacuum source can apply negative pressure to one side of the membrane to draw the agent therethrough.

Abstract: Modified Western blot membranes and methods of using same are provided which allow confirmation of Lyme disease and screening for at least one additional tick-borne disease. The membranes and methods of the present invention may thus be used to screen for the presence of tick-borne diseases which may be transferred along with Lyme disease. A Western blot assay may also be employed to confirm the presence of such additional tick-borne disease.

Abstract: The binding assay device of the present invention provides a removable cassette with an array of parallel channels for receiving multiple samples for assay. The cassette generally comprises a substantially planar cassette base with open channels formed in its bottom surface and a pair of channel extensions extending from its top surface which contain closed channels corresponding to and in fluid communication with open channels. The closed channels are further provided with openings at their termini for introduction of test samples into the cassette. In an assembled device, the cassette is disposed between a base plate and a slotted cover plate adapted for receipt of the channel extensions of the cassette. A resilient cushioning pad placed between the cassette base and base plate improves interchannel sealing and reduces the volume of the base channels and samples needed.

Abstract: A blot screening apparatus is formed of a plate having numerous, elongated, narrow, shallow, parallel, separated reaction channels formed in one surface. The channel openings are positioned over a treated membrane which reacts with fluids introduced into the various channels. An elongated, narrow, groove-like socket, formed in the opposite plate surface, overlaps and extends transversely over each of the opposite and portions of the channels. Holes formed in the plate communicate the opposite ends of each channel with the base of its overlapping socket so that fluid samples may be separately introduced into each channel through its holes. Manifold plugs that are shaped to closely fit within the sockets are removably positioned in, and frictionally sealed against, the walls of the sockets, but are spaced from their adjacent socket bases.

Abstract: A cover plate, having a lower, flat surface containing an array of numerous, parallel, closely spaced apart, downwardly opening channels, is releasably clamped upon a flat support plate surface. The channels each have inlet and outlet holes communicating with the upper surface of the cover plate. A paper-like, resilient cushion sheet is clamped between the plate surfaces and a paper-like membrane, carrying a pattern of antigen-like material, is arranged between the sheet and the cover plate channeled surface. The channels are very narrow, shallow and many times longer than their widths and are closed by the stripe-like portions of the membrane which overlie the channels. The cushion sheet is compressed between adjacent channels and bulges upwardly at each of the channels to thereby bulge the membrane stripe-like portions into dome-shaped cross-sections extending into the channels.

Abstract: A templet (10) is provided having a lower plate (11) which receives a carrier sheet having antigens immobilized thereon as transferred from an electrophoresis gel. An upper plate (12) having a plurality of parallel channels (22) formed therein, is placed over the lower plate such that the channels lie over the carrier sheet. The two plates (11, 12) are then pressed tightly together, as by engagement with screws (16) at the periphery of the plates. A bridge bar (28) extends over the central portion of the plate and pressure screws (30) are mounted to the bridge bar in position to engage and apply pressure to the top surface of the upper plate (12). Various antibody containing liquids are introduced into the various channels through holes (25) extending to the ends of the channels; the solutions are allowed to remain in contact with the antigens on the carrier sheet for a period of time, after which the liquid is withdrawn from the channels, and the plates separated.