Abstract: Provided herein are dermal fillers including decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed; as well as methods and uses thereof for cosmetic and/or reconstructive applications. Also provided are methods for preparing such dermal fillers, and dermal filler kits.

Abstract: The disclosure provides a bread-derived scaffold biomaterial for supporting cells, the scaffold comprising a bread crumb and wherein the bread crumb comprises a three-dimensional porous structure to support the cells. Further provided is a use of the scaffold and a method of making same.

Abstract: Provided herein are scaffold biomaterials including a decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure; wherein the decellularized plant or fungal tissue may optionally be at least partially coated or mineralized, wherein the scaffold biomaterial may optionally further include a protein-based hydrogel and/or a polysaccharide-based hydrogel, or both. Also provided herein are methods and uses of such scaffold biomaterials, including methods of manufacture as well as methods and uses for bone tissue engineering, for example.

Abstract: Provided herein are scaffold biomaterials including at least one bundle of microchannels, the bundle of microchannels having a plurality of decellularised microchannels isolated from plant or fungal tissue, the decellularised microchannels being arranged substantially parallel to each other within the bundle. Also provided are methods and uses of such scaffold biomaterials and bundles, as well as methods for the production of such scaffold biomaterials and bundles.

Abstract: Provided herein are composite scaffold biomaterials including two or more scaffold biomaterial subunits, each including a decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure, the two or more scaffold biomaterial subunits being assembled into the composite scaffold biomaterial and held together via gel casting using a hydrogel glue; via complementary interlocking geometry of the two or more scaffold biomaterial subunits; via guided assembly based biolithography (GAB); via chemical cross-linking; or any combinations thereof. Methods for producing such scaffold biomaterials, as well as methods and uses thereof, are also provided.

Abstract: The present invention provides methods of determining a characteristic of a cell, such as cell type, cellular response to a biochemical event, and biological state. The method generally involves detecting membrane movement in a cell to determine a characteristic of a cell. The methods of the invention are useful for applications such as drug screening and diagnostics. The invention further provides databases of cell characteristics, as determined by the instant methods. The invention further provides systems for determining the characteristic of a cell.