Abstract: The present invention includes a method and apparatus for the detection and analysis of information-containing signals in chromatographic data using iterative blind deconvolution and fuzzy logic algorithms. The invented method analyzes chromatographic data from a wide variety of sources of DNA sequencing information, including gel and capillary electrophoresis. Autoradiograms, single-fluor, four-lane and four fluor, single lane fluorescent chromatographic data are suitable sources of unprocessed input data. The output from the invented base calling method includes called (identified) sequence data and quality values for the called bases.