Abstract: Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 103 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Abstract: Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 103 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Abstract: A valve includes a bobbin/valve body, a solenoid and a plunger. The bobbin/valve body is formed in one piece, defines all of the valve's inlet/outlet ports and forms the entire support structure for the solenoid and the plunger. To assemble the valve, a plunger body is inserted into the bobbin/valve body through an end opening into a longitudinal bore and a spring positioned to bias the plunger body to a normally closed position. A pole piece is then inserted through the end opening into the longitudinal bore and attached to the bobbin/valve body. A solenoid coil is wound around a central cylindrical section of the bobbin/valve body, and terminal pins and a flux conductor are attached to the bobbin/valve body. The attachment of the pole piece, the terminal pins, and/or the flux conductor may be accomplished by press-fit and/or swage coupling techniques.

Abstract: A valve includes a bobbin/valve body, a solenoid and a plunger. The bobbin/valve body is formed in one piece, defines all of the valve's inlet/outlet ports and forms the entire support structure for the solenoid and the plunger. To assemble the valve, a plunger body is inserted into the bobbin/valve body through an end opening into a longitudinal bore and a spring positioned to bias the plunger body to a normally closed position. A pole piece is then inserted through the end opening into the longitudinal bore and attached to the bobbin/valve body. A solenoid coil is wound around a central cylindrical section of the bobbin/valve body, and terminal pins and a flux conductor are attached to the bobbin/valve body. The attachment of the pole piece, the terminal pins, and/or the flux conductor may be accomplished by press-fit and/or swage coupling techniques.

Abstract: A valve includes a bobbin/valve body, a solenoid and a plunger. The bobbin/valve body is formed in one piece, defines all of the valve's inlet/outlet ports and forms the entire support structure for the solenoid and the plunger. To assemble the valve, a plunger body is inserted into the bobbin/valve body through an end opening into a longitudinal bore and a spring positioned to bias the plunger body to a normally closed position. A pole piece is then inserted through the end opening into the longitudinal bore and attached to the bobbin/valve body. A solenoid coil is wound around a central cylindrical section of the bobbin/valve body, and terminal pins and a flux conductor are attached to the bobbin/valve body. The attachment of the pole piece, the terminal pins, and/or the flux conductor may be accomplished by press-fit and/or swage coupling techniques.