Abstract: The present disclosure provides recombinant defluorinating enzymes for the remediation of PFAs, PCBs, and other organo-halides, polynucleotides encoding the recombinant defluorinating enzymes, host cells expressing the recombinant defluorinating enzymes, and methods of using the recombinant defluorinating enzymes. Host cells can be engineered to express the recombinant defluorinating enzymes, for remediation of PFAs, PCBs, and other organo-halides.

Abstract: The present disclosure provides variant cytochrome P450 monooxygenase for the remediation of crude oil, PFAs, PCBs, and other organo-halides, polynucleotides encoding the variant cytochrome P450 monooxygenase, host cells expressing the variant cytochrome P450 monooxygenase, and methods of using the variant cytochrome P450 monooxygenase. Host cells can comprise the variant cytochrome P450 monooxygenase, almA (flavin binding monooxygenase), and xylE (catechol dioxygenase) for remediation of crude oil, PFAs, PCBs, and other organo-halides.

Abstract: A system may include a controller that may control operations of a device according to a control loop and characterize a frequency response of the device while the device is operating. The controller may characterize the frequency response by adding a perturbation signal to any signal in the control loop. The controller may then determine a first transformed signal by performing a first discrete Fourier transform on a first signal in the control loop at a frequency of the perturbation signal and determine a second transformed signal by performing a second discrete Fourier transform on a second signal in the control loop at the frequency of the perturbation signal. The controller may then determine the frequency response at the frequency by comparing a first amplitude and a first phase of the first transformed signal to a second amplitude and a second phase of the second transformed signal.

Abstract: A system may include a controller that may control operations of a device according to a control loop and characterize a frequency response of the device while the device is operating. The controller may characterize the frequency response by adding a perturbation signal to any signal in the control loop. The controller may then determine a first transformed signal by performing a first discrete Fourier transform on a first signal in the control loop at a frequency of the perturbation signal and determine a second transformed signal by performing a second discrete Fourier transform on a second signal in the control loop at the frequency of the perturbation signal. The controller may then determine the frequency response at the frequency by comparing a first amplitude and a first phase of the first transformed signal to a second amplitude and a second phase of the second transformed signal.

Abstract: An approach is disclosed for generating stereoscopic image sequences. The approach includes receiving a plurality of image sequences, each image sequence captured from a distinct camera or other capture device in a multi-camera array. One image sequence is a primary image sequence and the other image sequences are secondary image sequences. The approach further includes generating, from the primary and at least one or more of the secondary image sequences, depth maps corresponding to images of the primary image sequence and generating, for each image in the primary image sequence, based on at least the depth maps and the corresponding images of the primary image sequence, stereoscopic images of a stereoscopic image sequence.

Abstract: Techniques for creating 3-D movies allow improved control over camera positioning parameters and editing of depth in post-process to provide for a smoother variation in the viewer's convergence distance and a more pleasant viewing experience. A director can define reference parameters related to a desired viewing experience, and camera positioning parameters are derived therefrom. A depth script specifying piecewise continuous variations in reference parameters can be applied in post-process to generate 3-D shots, scenes, or movies. These techniques can be applied in both computer-generated and live-action 3-D movies.

Abstract: Techniques for creating 3-D movies allow improved control over camera positioning parameters and editing of depth in post-process to provide for a smoother variation in the viewer's convergence distance and a more pleasant viewing experience. A director can define reference parameters related to a desired viewing experience, and camera positioning parameters are derived therefrom. A depth script specifying piecewise continuous variations in reference parameters can be applied in post-process to generate 3-D shots, scenes, or movies. These techniques can be applied in both computer-generated and live-action 3-D movies.

Abstract: An approach is disclosed for generating stereoscopic image sequences. The approach includes receiving a plurality of image sequences, each image sequence captured from a distinct camera or other capture device in a multi-camera array. One image sequence is a primary image sequence and the other image sequences are secondary image sequences. The approach further includes generating, from the primary and at least one or more of the secondary image sequences, depth maps corresponding to images of the primary image sequence and generating, for each image in the primary image sequence, based on at least the depth maps and the corresponding images of the primary image sequence, stereoscopic images of a stereoscopic image sequence.

Abstract: Techniques for creating 3-D movies allow improved control over camera positioning parameters and editing of depth in post-process to provide for a smoother variation in the viewer's convergence distance and a more pleasant viewing experience. A director can define reference parameters related to a desired viewing experience, and camera positioning parameters are derived therefrom. A depth script specifying piecewise continuous variations in reference parameters can be applied in post-process to generate 3-D shots, scenes, or movies. These techniques can be applied in both computer-generated and live-action 3-D movies.

Abstract: In general, the invention consists of a means for staining a sample with a fluorescent dye, a means for producing a darkfield image of the fluorescent stained sample, and a means of transforming an image of a sample stained with one or more darkfield dyes into an image stained with one or more brightfield dyes for examination on a computer monitor. The process includes applying a digital lookup table or other computational means in order to convert the darkfield data to brightfield forms, and a means of displaying said transformed information. Preferably, the imaging means is a block face microscope, and the means for transforming the images is a digital computer.

Abstract: In general, the invention consists of a means for staining a sample with a fluorescent dye, a means for producing a darkfield image of the fluorescent stained sample, and a means of transforming an image of a sample stained with one or more darkfield dyes into an image stained with one or more brightfield dyes for examination on a computer monitor. The process includes applying a digital lookup table or other computational means in order to convert the darkfield data to brightfield forms, and a means of displaying said transformed information. Preferably, the imaging means is a block face microscope, and the means for transforming the images is a digital computer.