Patents by Inventor Andrew J. Blasband
Andrew J. Blasband has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7595189Abstract: The device for detecting the binding of two chemical species includes a first plate having a base, multiple optical fibers and a second plate. The base has multiple grooves formed therein. The multiple optical fibers are each disposed within a corresponding one of the multiple grooves. The second plate has multiple channels formed therein. The first plate and the second plate are configured to be placed adjacent to one another such that each the optical fiber is exposed to and traverses the multiple channels.Type: GrantFiled: June 24, 2004Date of Patent: September 29, 2009Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Tim Geiser, Andrew J. Blasband
-
Patent number: 7414115Abstract: Disclosed is a kit useful for determining the number of repeat units in a repeat region of a target nucleic acid comprising: a plurality of different-sequence primers, each containing (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment, a first primer extension reagent; and a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a photodestructible label attached thereto.Type: GrantFiled: March 30, 2007Date of Patent: August 19, 2008Assignee: Applied Biosystems Inc.Inventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Patent number: 7294464Abstract: Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.Type: GrantFiled: July 14, 2004Date of Patent: November 13, 2007Assignee: Applera CorporationInventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Patent number: 6982149Abstract: An apparatus and method are provided for contacting at least two chemical species, comprising a support plate having a channel for receiving a mobile chemical species and a fiber, having a second chemical species immobilized thereon, disposed on the support plate. At least a portion of the fiber is exposed to the channel such that the mobile chemical species is capable of contacting the second chemical species. An apparatus and method for reading the fiber array, an apparatus and method for making the fiber array, and methods of using the fiber array of the present invention are also provided.Type: GrantFiled: June 23, 2003Date of Patent: January 3, 2006Assignee: Applera CorporationInventors: Charles S. Vann, Tim Geiser, Andrew J. Blasband
-
Patent number: 6773887Abstract: Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.Type: GrantFiled: October 22, 2001Date of Patent: August 10, 2004Assignee: Applera CorporationInventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Publication number: 20040126756Abstract: Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.Type: ApplicationFiled: October 22, 2001Publication date: July 1, 2004Applicant: PE Corporation (NY)Inventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Publication number: 20030232381Abstract: An apparatus and method are provided for contacting at least two chemical species, comprising a support plate having a channel for receiving a mobile chemical species and a fiber, having a second chemical species immobilized thereon, disposed on the support plate. At least a portion of the fiber is exposed to the channel such that the mobile chemical species is capable of contacting the second chemical species. An apparatus and method for reading the fiber array, an apparatus and method for making the fiber array, and methods of using the fiber array of the present invention are also provided.Type: ApplicationFiled: June 23, 2003Publication date: December 18, 2003Inventors: Charles S. Vann, Tim Geiser, Andrew J. Blasband
-
Patent number: 6649404Abstract: The invention provides a method for contacting at least two chemical species by immobilizing an immobilized chemical species on a fiber, placing the fiber on a support having a channel, and disposing a mobile chemical species into the channel such that the immobilized chemical species contacts the fiber. The invention also provides methods for analyzing the contact between at least two chemical species, detecting the binding of two chemical species, making a microchip, and synthesizing a chemical species on a fiber.Type: GrantFiled: June 8, 2000Date of Patent: November 18, 2003Assignee: Applera CorporationInventors: Charles S. Vann, Tim Geiser, Andrew J. Blasband
-
Patent number: 6451525Abstract: A method for obtaining sequence information from a plurality of target polynucleotides in a sample is disclosed. In one embodiment, the method involves contacting a plurality of different sequence primers with a polynucleotide sample under conditions effective for the primers to anneal to primer-complementary regions in one or more target polynucleotides, to form one or more target-primer hybrid(s). Each different-sequence primer contains (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment. The hybrid(s) are contacted with a labeled nucleotide terminator in the presence of a primer-extending reagent, under conditions effective to append the base to an end of the annealed primer in the hybrid only when the base is complementary to a base in the target polynucleotide that is immediately adjacent to the end of the annealed primer, to form an extended hybrid mixture.Type: GrantFiled: December 3, 1998Date of Patent: September 17, 2002Assignee: PE Corporation (NY)Inventors: Andrew J. Blasband, Sandy M. Koepf, Adam L. Lowe
-
Patent number: 6309829Abstract: Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.Type: GrantFiled: December 3, 1998Date of Patent: October 30, 2001Assignee: PE Corporation (NY)Inventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Patent number: 5945284Abstract: In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.Type: GrantFiled: May 27, 1997Date of Patent: August 31, 1999Assignee: The Perkin-Elmer CorporationInventors: Kenneth J. Livak, Adam L. Lowe, Andrew J. Blasband
-
Patent number: 5384025Abstract: The present invention is directed to improved side spacers for use in slab gel electrophoresis that prevent the formation of channels between the electrophoresis gel and the side spacer and methods employing the side spacer. The improvement consists of forming the side spacer with one or more notches cut into the edge of the side spacer in contact with the gel, such that, when the gel hardens, the side spacer is anchored into the gel, thereby preventing the formation of channels between the side spacer and the gel.Type: GrantFiled: March 7, 1994Date of Patent: January 24, 1995Assignee: Applied Biosystems, Inc.Inventor: Andrew J. Blasband