Patents by Inventor Andrew J. G. Simpson

Andrew J. G. Simpson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for purification of a fatty acid binding protein
    Patent number: 9475838
    Abstract: A process for purification of a fatty acid binding proteins such as, e.g., Sm14 of Pichia pastoris or type-3 FABP protein of Fasciola hepatica. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: October 25, 2016
    Assignees: OURO FINO AGRONEGOCIO LTDA., FUNDACAO OSWALDO CRUZ
    Inventors: Miriam Tendler, Celso Raul Romero Ramos, Andrew J. G. Simpson
  • Synthetic gene for expressing Sm-14 in , methods for producing and purifying Sm-14 and the use thereof as a vaccine and diagnostic medium
    Patent number: 9193772
    Abstract: The production of recombinant proteins using a synthetic gene for increased expression of the protein in Pichia pastoris is disclosed. More specifically, disclosed is a process for producing a recombinant Sm14 protein of Schistosoma mansoni, by providing a synthetic gene for increased production of Sm14 protein, producing the gene and genetically manipulating the Pichia pastoris strain with the gene in order to produce a vaccine effectively. Improved methods are also provided for producing and purifying this protein from P. pastoris cells, which can be scaled for industrial production.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: November 24, 2015
    Assignees: ALVOS—CONSULTORIA, DESENVOLVIMENTO E COMERCIALIZAçÃO DE PRODUTOS BIOTECNOLÓGICOS S/A, FUNDAçÃO OSWALDO CRUZ
    Inventors: Miriam Tendler, Celso Raul Romero Ramos, Andrew J. G. Simpson
  • Staphylococcus aureus genes and polypeptides
    Patent number: 6821754
    Abstract: The present invention relates to 11 novel genes from S. aureus and the polypeptides they encode. Also provided as are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of S. aureus polypeptide activity. The invention additionally relates to diagnostic methods for detecting Staphylococcus nucleic acids, polypeptides and antibodies in a biological sample. The present invention further relates to novel vaccines for the prevention or attenuation of infection by Staphylococcus.
    Type: Grant
    Filed: October 24, 2002
    Date of Patent: November 23, 2004
    Assignees: Human Genome Sciences, Inc., Ludwig Institute for Cancer Research
    Inventors: Andrew J. G. Simpson, Gil H. Choi
  • Staphylococcus aureus genes and polypeptides
    Patent number: 6521441
    Abstract: The present invention relates to 11 novel genes from S. aureus and the polypeptides they encode. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of S. aureus polypeptide activity. The invention additionally relates to diagnostic methods for detecting Staphylococcus nucleic acids, polypeptides and antibodies in a biological sample. The present invention further relates to novel vaccines for the prevention or attenuation of infection by Staphylococcus.
    Type: Grant
    Filed: January 15, 2002
    Date of Patent: February 18, 2003
    Assignees: Human Genome Sciences, Inc., Ludwig Institute For Cancer Research
    Inventors: Andrew J. G. Simpson, Gil H. Choi
  • Fingerprinting using single specific primers in low stringency polymerase chain reaction conditions
    Patent number: 5599674
    Abstract: A straightforward method to characterize a nucleotide sequence of a defined DNA region is described. A purified segment representing the defined region is subjected to repeated cycles of amplification (PCR) using a single primer complementary to a portion of the segment under conditions of low stringency and high polymerase and primer concentrations. PCR under these conditions results in a mixture of amplified DNA molecules the composition of which is characteristic of the DNA segment assessed. Determination of the composition of the mixture can be obtained by subjecting the mixture to standard chromatographic or electrophoretic techniques to obtain a pattern of fragments which represents a "signature" of the nucleotide sequence.
    Type: Grant
    Filed: March 20, 1995
    Date of Patent: February 4, 1997
    Assignee: Sergio D. J. Pena
    Inventors: Sergio D. J. Pena, Andrew J. G. Simpson