Patents by Inventor Andrew John Heron
Andrew John Heron has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11965183Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: October 13, 2021Date of Patent: April 23, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
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Publication number: 20240124915Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.Type: ApplicationFiled: February 17, 2023Publication date: April 18, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Clive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
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Publication number: 20240076729Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.Type: ApplicationFiled: June 18, 2021Publication date: March 7, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark John Bruce, Daniel Ryan Garalde, James Edward Graham, Andrew John Heron, Etienne Raimondeau, James White, Christopher Peter Youd
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Patent number: 11920193Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: GrantFiled: June 6, 2019Date of Patent: March 5, 2024Assignee: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Johnson, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
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Patent number: 11921103Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.Type: GrantFiled: June 21, 2019Date of Patent: March 5, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, James Anthony Clarke, Andrew John Heron
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Publication number: 20240060126Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein.Type: ApplicationFiled: June 27, 2023Publication date: February 22, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
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Publication number: 20240044865Abstract: Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.Type: ApplicationFiled: July 24, 2023Publication date: February 8, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Edward Graham, Melania Slawa Strycharska
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Publication number: 20240026441Abstract: The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.Type: ApplicationFiled: June 23, 2023Publication date: January 25, 2024Applicant: Oxford Nanopre Technologies PLCInventors: Andrew John Heron, Clive Gavin Brown, Rebecca Victoria Bowen, James White, Daniel John Turner, Joseph Hargreaves Lloyd, Christopher Peter Youd
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Patent number: 11845780Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.Type: GrantFiled: December 1, 2020Date of Patent: December 19, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20230357821Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.Type: ApplicationFiled: February 17, 2023Publication date: November 9, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Clive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
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Publication number: 20230295712Abstract: The invention provides a method of selectively characterising polynucleotides of a desired property, such as length, using a nanopore, based on the translocation of the polynucleotide through or across the nanopore. Kits and systems for use in such methods are also provided. The methods of the invention are particularly suitable for sequencing polynucleotides such as DNA.Type: ApplicationFiled: June 18, 2021Publication date: September 21, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark John Bruce, Daniel Ryan Garalde, James Edward Graham, Andrew John Heron, Etienne Raimondeau, James White, Christopher Peter Youd
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Patent number: 11739377Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.Type: GrantFiled: October 20, 2020Date of Patent: August 29, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
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Patent number: 11739379Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: GrantFiled: June 29, 2021Date of Patent: August 29, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Edward Graham, Richard Alexander Gutierrez, Rebecca Victoria Bowen, James White, Clive Gavin Brown, Daniel George Fordham
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Patent number: 11725235Abstract: The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.Type: GrantFiled: August 14, 2019Date of Patent: August 15, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, Clive Gavin Brown, Rebecca Victoria Bowen, James White, Daniel John Turner, Joseph Hargreaves Lloyd, Christopher Peter Youd
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Publication number: 20230227799Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 18, 2022Publication date: July 20, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark John Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, David Antoni Alves, Rebecca Victoria Bowen, John Milton
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Publication number: 20230227902Abstract: Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.Type: ApplicationFiled: June 18, 2021Publication date: July 20, 2023Applicant: Oxford Nanopore Technologies PLCInventors: James Edward Graham, Christopher Peter Youd, Clive Gavin Brown, Marion Louise Crawford, Andrew John Heron, James White
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Publication number: 20230227903Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.Type: ApplicationFiled: June 18, 2021Publication date: July 20, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark John Bruce, Daniel Ryan Garalde, James Edward Graham, Andrew John Heron, Etienne Raimondeau, James White, Christopher Peter Youd
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Publication number: 20230212535Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 17, 2022Publication date: July 6, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Mark Bruce, Andrew John Heron, Ruth Moysey, Szabolcs Soeroes, Elizabeth Jayne Wallace, James White
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Publication number: 20230212665Abstract: The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.Type: ApplicationFiled: December 14, 2022Publication date: July 6, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, David Antoni Alves, James Anthony Clarke, Marion Louise Crawford, Daniel Ryan Garalde, Graham Hall, Daniel John Turner, James White
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Publication number: 20230167494Abstract: A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.Type: ApplicationFiled: June 28, 2022Publication date: June 1, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, Daniel John Turner, Andrew John Heron, Christopher James Wright