Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract: A portable, self-contained beverage apparatus includes a container assembly having a known storage capacity for storing a consumable liquid, and a dispensing assembly disposed within the container assembly that dispenses variable, non-zero quantities of additives into the consumable liquid. The dispensing assembly includes multiple apertures structured and arranged to retain vessels containing the additives to be dispensed into the consumable liquid. The beverage apparatus also includes a level sensor disposed within the container assembly that determines a consumable liquid level of the consumable liquid stored in the container assembly. In certain embodiments, one or more positive displacement pumping mechanisms are configured to pump additive liquid from additive containers into a beverage chamber.

Abstract: Failure detection and reporting for a computer mail gateway is provided. In a preferred embodiment, two computer mail systems are connected by a gateway. The gateway includes an automatic gateway failure detection and reporting facility. The gateway also includes a message transfer store. The facility transmits a message from the first mail system into the message transfer store for retrieval by the second mail system. The facility then detects that the transmitted message from the first mail system has remained in the message transfer store for at least a predetermined time without being retrieved by the second mail system. In response to detecting that the transmitted message from the first mail system has remained in the message transfer store for at least a predetermined time without being retrieved by the second mail system, the facility diagnoses the failure of the gateway.