Abstract: A silencer comprises a cylindrical outer housing with a rifle coupling end, a cylindrical inner housing positioned within a portion of the cylindrical outer housing, a deflector within the cylindrical inner housing and with a semispherical surface with a central opening forming a hollow bore, wherein some of the expanding gas ejecta behind the fired projectile is retained by deflector and directed through a longer and convoluted escape path, and at least one series of stacked cone shaped baffles within the cylindrical inner housing each with a central opening forming a hollow bore and wherein some of the expanding gas ejecta behind the fired projectile is retained by baffles and directed through a longer and convoluted escape path, and wherein the structure of the silencer slows down the gas ejecta behind the fired projectile and dissipates its kinetic energy.

Abstract: An automated medication administration system that is employed with an integrated cartridge assembly in response to the acute medical condition. The pump assembly is configured to engage the cartridge assembly having at least a first container containing saline and a second container containing a medicament. The engagement of the cartridge assembly transitions a gate member of the cartridge assembly from a locked position to an unlocked position. The pump includes a drive assembly that is configured to convey repeated doses of the medicament in accordance with a treatment protocol when the gate member is in the unlocked position. Each dose of the medicament is followed by a dose of the saline.

Abstract: An integrated cartridge assembly that is employed with an automated medication administration system in response to the acute medical condition. The cartridge assembly configured to be operatively coupled between a pump assembly and a medical port. The cartridge assembly includes a first cartridge and a second cartridge positioned within a housing. The first cartridge contains a portion of saline, while the second cartridge contains a portion of a medicament. The cartridge assembly also includes a gate member that maintains a separation distance between a manifold assembly and the first and second cartridges when in a locked position when the cartridge assembly is in the stored state. The cartridge assembly is operatively coupled to the medical port via a tube set that is fixedly coupled to the manifold assembly.

Abstract: Disclosed herein, inter alia, are compositions and methods for amplification and sequencing of nucleic acids.

Abstract: An integrated cartridge assembly that is employed with an automated medication administration system in response to the acute medical condition. The cartridge assembly configured to be operatively coupled between a pump assembly and a medical port. The cartridge assembly includes a first cartridge and a second cartridge positioned within a housing. The first cartridge contains a portion of saline, while the second cartridge contains a portion of a medicament. The cartridge assembly also includes a gate member that maintains a separation distance between a manifold assembly and the first and second cartridges when in a locked position when the cartridge assembly is in the stored state. The cartridge assembly is operatively coupled to the medical port via a tube set that is fixedly coupled to the manifold assembly.

Abstract: Disclosed herein, inter alia, are compositions and methods for amplification of nucleic acid templates, including hybridizing a linear polynucleotide to an immobilized primer on a surface, circularizing the linear polynucleotide to form a circular polynucleotide, and extending the primer with a polymerase.

Abstract: An integrated cartridge assembly that is employed with an automated medication administration system in response to the acute medical condition. The cartridge assembly configured to be operatively coupled between a pump assembly and a medical port. The cartridge assembly includes a first cartridge and a second cartridge positioned within a housing. The first cartridge contains a portion of saline, while the second cartridge contains a portion of a medicament. The cartridge assembly also includes a gate member that maintains a separation distance between a manifold assembly and the first and second cartridges when in a locked position when the cartridge assembly is in the stored state. The cartridge assembly is operatively coupled to the medical port via a tube set that is fixedly coupled to the manifold assembly.

Abstract: Techniques for dynamically converting a communication channel associated with a communication channel from a communication channel associated with a single organization to a shared communication channel associated with two or more organizations. The communication channel may have associated therewith member profiles associated with a first organization and one or more guest profiles associated with one or more other organizations that are different from the first organization. A server computing system may convert the communication channel to a shared communication channel based on a determination of eligibility for conversion. The server computing system may generate one or more user interfaces associated with the member profiles and the one or more guest profiles, enabling members and guests to communicate via the shared communication channel.

Abstract: Methods, apparatus and computer program product that may include receiving, by one or more servers of a group-based communication platform and from a client of a first user with administrative privileges and associated with a first organization, a request to generate a shared communication channel on the group-based communication platform. A shared communication channel may be generated based on the request. A second request may be received by the one or more servers to provide at least one second user associated with a second organization access to the shared communication channel. Access may be provided to the second user. A message communication may be rendered in a shared communication channel interface associated with the shared communication channel with a graphical indicator that indicates the message communication is associated with the first organization.

Abstract: Disclosed herein, inter alia, are compositions and methods for efficient amplification and sequencing of nucleic acid templates.

Abstract: The present system incorporates mobile computing devices to play games, such as electronic pull-tabs, in a plurality of venues, each having a local wireless network. Each network communicates with a central system that generates decks of game outcomes and associated awards. All wagers and awards for each game are tracked on the central system, which also provides each game award and associated outcome from a deck stored on the central system.

Abstract: Disclosed herein, in some embodiments, non-naturally occurring proteins (e.g., non-naturally occurring modified proteins) that may be useful in the treatment of bacterial and viral infections, including SARS-CoV-2 infection, host cells comprising the same, and methods of treating bacterial and viral infections including SARS-CoV-2 infection. Also provided herein are host cells comprising fusion proteins for split intein-based selection of peptides that bind a target protein, methods of using the same, and methods of identifying peptides that bind a target protein.

Abstract: Disclosed herein, inter alia, are methods for sequencing both strands of a double stranded nucleic acid fragment. Compositions and kits for use in the methods are also provided.

Abstract: The present system incorporates mobile computing devices to play games, such as electronic pull-tabs, in a plurality of venues, each having a local wireless network. Each network communicates with a central system that generates decks of game outcomes and associated awards. All wagers and awards for each game are tracked on the central system, which also provides each game award and associated outcome from a deck stored on the central system.

Abstract: Methods, apparatus and computer program product for causing a rendering of a graphical icon associated with a message communication, wherein the message communication is received in a shared communication channel in a group-based communication platform. The graphical icon comprises a user avatar associated with a sender of the message communication and a group icon indicating association of the message communication with a group.

Abstract: Computing systems, apparatuses, computer-implemented methods, and computer program products are disclosed for creating a shared communication channel in a group-based communication platform having a plurality of database shards. An example computer-implemented method includes generating a shared communication channel shard that is assigned a shared communication channel identification, a first set of shared communication channel attributes associated with a first group identification, and a second set of shared communication channel attributes associated with a second group identification. The method further includes generating first and second externally shared group-based shared communication channel interfaces based on the first and second sets of shared communication channel attributes, respectively.

Abstract: Techniques for dynamically converting a communication channel associated with a communication channel from a communication channel associated with a single organization to a shared communication channel associated with two or more organizations. The communication channel may have associated therewith member profiles associated with a first organization and one or more guest profiles associated with one or more other organizations that are different from the first organization. A server computing system may convert the communication channel to a shared communication channel based on a determination of eligibility for conversion. The server computing system may generate one or more user interfaces associated with the member profiles and the one or more guest profiles, enabling members and guests to communicate via the shared communication channel.

Abstract: Methods, apparatus and computer program product for causing a rendering of a graphical icon associated with a message communication, wherein the message communication is received in a shared communication channel in a group-based communication platform. The graphical icon comprises a user avatar associated with a sender of the message communication and a group icon indicating association of the message communication with a group.

Abstract: A trailer monitoring system for monitoring a trailer having at least one trailer sensor has a bridge integrator unit, configured to couple with the trailer, including a wireless data transceiver electrically coupled with a microcontroller and capable of wirelessly communicating data over a short-range wireless protocol; the bridge integrator unit receives data existing in a first messaging format that is compatible with and used by one or more trailer sensors via a short-range wireless protocol, extracts the data, encodes the data into a second messaging format that is compatible with a master control unit having a telematic data transceiver capable of cellular data communications, and transmits the data in the second messaging format from the bridge integrator unit to the master control unit via a short-range wireless protocol.

Abstract: Techniques for dynamically converting a communication channel associated with a communication channel from a communication channel associated with a single organization to a shared communication channel associated with two or more organizations. The communication channel may have associated therewith member profiles associated with a first organization and one or more guest profiles associated with one or more other organizations that are different from the first organization. A server computing system may convert the communication channel to a shared communication channel based on a determination of eligibility for conversion. The server computing system may generate one or more user interfaces associated with the member profiles and the one or more guest profiles, enabling members and guests to communicate via the shared communication channel.