Abstract: A multi-mode control device is provided for controlling an external load device. The control device includes a high-power interface, a low-power interface, and a control module. The high-power interface can be electrically coupled to a high-power module providing current from an external power source to the load device. The low-power interface can be electrically coupled to a low-power module. The high-power interface can receive a first current from the high-power module. The low-power interface can receive a second current from the low-power module that is less than the first current. The low-power interface can prevent the first current from flowing to the low-power module. The control module, which is electrically coupled to the high-power interface and the low-power interface, be powered by one or more of the first and second current.

Abstract: Agents that specifically bind to a muscarinic acetylcholine receptor in a conformationally specific way can be used to induce a conformational change in the receptor. Such agents have therapeutic applications and can be used in X-ray crystallography studies of the receptor. Such agents can also be used to improve drug discovery via compound screening and/or structure based drug design.

Abstract: Provided herein are several methods for selecting agents that bind to transmembrane receptors in a conformationally-selective way. In some embodiments, the method may comprise producing: a transmembrane receptor in an active conformation; and said transmembrane receptor in an inactive conformation and using cell sorting to select, from a population of cells comprising a library of cell surface-tethered extracellular capture agents, cells that are specifically bound to either the transmembrane receptor in its active conformation or the transmembrane receptor in its inactive conformation, but not both. In other embodiments, the method may comprise: contacting a GPCR with a population of cells that comprise a library of surface-tethered extracellular proteins; labeling the cell population with a conformationally-specific binding agent, e.g., a G-protein or mimetic thereof; and using cell sorting to select from the cell population cells that bind to the agent.

Abstract: A multi-mode control device is provided for controlling an external load device. The control device includes a high-power interface, a low-power interface, and a control module. The high-power interface can be electrically coupled to a high-power module providing current from an external power source to the load device. The low-power interface can be electrically coupled to a low-power module. The high-power interface can receive a first current from the high-power module. The low-power interface can receive a second current from the low-power module that is less than the first current. The low-power interface can prevent the first current from flowing to the low-power module. The control module, which is electrically coupled to the high-power interface and the low-power interface, be powered by one or more of the first and second current.

Abstract: Methods and compositions are provided for displaying a protein of interest (POI) on the surface of a eukaryotic cell by fusing the POI to a signal polypeptide, a stalk polypeptide, and a surface anchor polypeptide to generate a surface accessible fusion protein. Nucleic acids are provided that include nucleotide sequences encoding a signal polypeptide, a stalk polypeptide, and a surface anchor polypeptide. In some cases, a subject nucleic acid includes and insertion site for the insertion of a POI. In some cases, a subject nucleic acid includes a nucleotide sequence that encodes a POI. In some cases a stalk polypeptide is a synthetic stalk polypeptide and various example synthetic stalk polypeptides are disclosed. In some cases, a surface anchor polypeptide is a glycosylphosphatidylinisotol (GPI) anchor domain, which can be synthetic. Kits are also provided for practicing the subject methods.

Abstract: Provided herein are several methods for selecting agents that bind to transmembrane receptors in a conformationally-selective way. In some embodiments, the method may comprise producing: a transmembrane receptor in an active conformation; and said transmembrane receptor in an inactive conformation and using cell sorting to select, from a population of cells comprising a library of cell surface-tethered extracellular capture agents, cells that are specifically bound to either the transmembrane receptor in its active conformation or the transmembrane receptor in its inactive conformation, but not both. In other embodiments, the method may comprise: contacting a GPCR with a population of cells that comprise a library of surface-tethered extracellular proteins; labeling the cell population with a conformationally-specific binding agent, e.g., a G-protein or mimetic thereof; and using cell sorting to select from the cell population cells that bind to the agent.

Abstract: A multi-mode control device is provided for controlling an external load device. The control device includes a high-power interface, a low-power interface, and a control module. The high-power interface can be electrically coupled to a high-power module providing current from an external power source to the load device. The low-power interface can be electrically coupled to a low-power module. The high-power interface can receive a first current from the high-power module. The low-power interface can receive a second current from the low-power module that is less than the first current. The low-power interface can prevent the first current from flowing to the low-power module. The control module, which is electrically coupled to the high-power interface and the low-power interface, can operate in a high-power mode for powering the control module using the first current and a low-power mode for powering the control module using the lower second current.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: A multi-mode control device is provided for controlling a load device. A high-power interface of the control device can be electrically coupled to a high-power module. An occupancy sensor can receive a first current from the high-power module via the high-power interface, and a trigger detection device can receive a second current that is less than the first current from a low-power module via a low-power interface. The processor can switch the control device from a high-power mode for powering the occupancy sensor to a low-power mode by causing a reduction in the current provided to the occupancy sensor and causing current to be provided to the trigger detection device. The trigger detection device can detect a trigger in the low-power mode. The processor can cause the control device to operate in the high-power mode based on the trigger's detection.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: A multi-mode control device is provided for controlling a load device. A high-power interface of the control device can be electrically coupled to a high-power module. An occupancy sensor can receive a first current from the high-power module via the high-power interface, and a trigger detection device can receive a second current that is less than the first current from a low-power module via a low-power interface. The processor can switch the control device from a high-power mode for powering the occupancy sensor to a low-power mode by causing a reduction in the current provided to the occupancy sensor and causing current to be provided to the trigger detection device. The trigger detection device can detect a trigger in the low-power mode. The processor can cause the control device to operate in the high-power mode based on the trigger's detection.

Abstract: A multi-mode control device is provided for controlling a load device. A high-power interface of the control device can be electrically coupled to a high-power module for providing current to the load device. An occupancy sensor can receive a first current from the high-power module via the high-power interface, and a trigger detection device can receive a second current that is less than the first current from a low-power module via a low-power interface. The processor can switch the control device from a high-power mode for powering the occupancy sensor to a low-power mode by causing a reduction in the current provided to the occupancy sensor and causing current to be provided to the trigger detection device. The trigger detection device can detect a trigger in the low-power mode. The processor can cause the control device to operate in the high-power mode based on the trigger's detection.

Abstract: A system and apparatus assists pilots and flight crews in determining the best course of action at any particular point inflight for any category of emergency. The system monitors a plurality of static and dynamic flight parameters including atmospheric conditions along the flight path, ground conditions and terrain, conditions aboard the aircraft, and pilot/crew data. Based on these parameters, the system may provide continually updated information to the pilot or crew about the best available landing sites or recommend solutions to aircraft configuration errors. In case of emergency, the system may provide the pilot with procedure sets associated with a hierarchy of available emergency landing sites (or execute these procedure sets via the autopilot system) depending on the specific nature of the emergency.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: Retrofit light fixtures suitable for installation without tools. One or more spring bands deform as the fixture is forced through a ceiling opening and resume their preloaded shape to hold the fixture in place. Some embodiments may be installed in round ceiling openings and may utilize LED light sources.

Abstract: Agents that specifically bind to a muscarinic acetylcholine receptor in a conformationally-specific way can be used to induce a conformational change in the receptor. Such agents have therapeutic applications and can be used in X-ray crystallography studies of the receptor. Such agents can also be used to improve drug discovery via compound screening and/or structure based drug design.

Abstract: Provided herein are several methods for selecting agents that bind to transmembrane receptors in a conformationally-selective way. In some embodiments, the method may comprise producing: a transmembrane receptor in an active conformation; and said transmembrane receptor in an inactive conformation and using cell sorting to select, from a population of cells comprising a library of cell surface-tethered extracellular capture agents, cells that are specifically bound to either the transmembrane receptor in its active conformation or the transmembrane receptor in its inactive conformation, but not both. In other embodiments, the method may comprise: contacting a GPCR with a population of cells that comprise a library of surface-tethered extracellular proteins; labeling the cell population with a conformationally-specific binding agent, e.g., a G-protein or mimetic thereof; and using cell sorting to select from the cell population cells that bind to the agent.

Abstract: A multi-mode control device is provided for controlling an external load device. The control device includes a high-power interface, a low-power interface, and a control module. The high-power interface can be electrically coupled to a high-power module providing current from an external power source to the load device. The low-power interface can be electrically coupled to a low-power module. The high-power interface can receive a first current from the high-power module. The low-power interface can receive a second current from the low-power module that is less than the first current. The low-power interface can prevent the first current from flowing to the low-power module. The control module, which is electrically coupled to the high-power interface and the low-power interface, can operate in a high-power mode for powering the control module using the first current and a low-power mode for powering the control module using the lower second current.