Patents by Inventor Andrew Laszlo

Andrew Laszlo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210293748
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Application
    Filed: March 15, 2021
    Publication date: September 23, 2021
    Applicant: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Publication number: 20210172013
    Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.
    Type: Application
    Filed: December 3, 2020
    Publication date: June 10, 2021
    Applicant: University of Washington through its Center for Commercialization
    Inventors: Jens H. Gundlach, Andrew Laszlo
  • Patent number: 10948454
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: March 16, 2021
    Assignee: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Publication number: 20210032695
    Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.
    Type: Application
    Filed: September 22, 2020
    Publication date: February 4, 2021
    Applicants: University of Washington through its Center for Commercialization, Illumina, Inc.
    Inventors: Jens H. Gundlach, Andrew Laszlo, Ian Derrington, Jeffrey G. Mandell
  • Patent number: 10858700
    Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: December 8, 2020
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Jens H. Gundlach, Andrew Laszlo
  • Publication number: 20200377936
    Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.
    Type: Application
    Filed: March 19, 2020
    Publication date: December 3, 2020
    Applicant: University of Washington through its Center for Commercialization
    Inventors: Jens Gundlach, Ian M. Derrington, Andrew Laszlo, Elizabeth Manrao
  • Patent number: 10822652
    Abstract: The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind in a re-analyte polymer that results in a polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.
    Type: Grant
    Filed: July 6, 2018
    Date of Patent: November 3, 2020
    Assignees: University of Washington through its Center for Commercialization, Illumina, Inc.
    Inventors: Jens H. Gundlach, Andrew Laszlo, Ian Derrington, Jeffrey G. Mandell
  • Patent number: 10612083
    Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: April 7, 2020
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Jens Gundlach, Ian M. Derrington, Andrew Laszlo, Elizabeth Manrao
  • Publication number: 20200049656
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Application
    Filed: July 22, 2019
    Publication date: February 13, 2020
    Applicant: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Patent number: 10359395
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: July 23, 2019
    Assignee: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Publication number: 20190106740
    Abstract: The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind in a re-analyte polymer that results in a polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.
    Type: Application
    Filed: July 6, 2018
    Publication date: April 11, 2019
    Applicants: University of Washington through its Center for Commercialization, Illumina, Inc.
    Inventors: Jens H. Gundlach, Andrew Laszlo, Ian Derrington, Jeffrey G. Mandell
  • Patent number: 10017814
    Abstract: The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.
    Type: Grant
    Filed: September 2, 2014
    Date of Patent: July 10, 2018
    Assignees: University of Washington through its Center for Commercialization, Illumina, Inc.
    Inventors: Jens H. Gundlach, Andrew Laszlo, Ian Derrington, Jeffrey G. Mandell
  • Publication number: 20170199149
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Application
    Filed: November 26, 2014
    Publication date: July 13, 2017
    Applicant: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Publication number: 20160222444
    Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.
    Type: Application
    Filed: September 2, 2014
    Publication date: August 4, 2016
    Applicants: University of Washington through its Center for Commercialization, Illumina, Inc.
    Inventors: Jens H. Gundlach, Andrew Laszlo, Ian Derrington, Jeffrey G. Mandell
  • Publication number: 20150191782
    Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.
    Type: Application
    Filed: August 2, 2013
    Publication date: July 9, 2015
    Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Jens H. Gundlach, Andrew Laszlo
  • Publication number: 20150132745
    Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Applicant: University of Washington Through its Center for Commercialization
    Inventors: Jens Gundlach, Ian M. Derrington, Andrew Laszlo, Elizabeth Manrao
  • Patent number: 7640037
    Abstract: A method of processing information received in the form of a message from a mobile handheld device with a camera, where the message includes data representing an image of a document and an identifier of a type of said document. The method preferably provides image enhancement, information recognition and analysis selected based on the identified document type. An output of the system is preferably provided to the user in the form specified by the user, such as an e-mail.
    Type: Grant
    Filed: May 18, 2005
    Date of Patent: December 29, 2009
    Assignee: scanR, Inc,
    Inventors: Andrew H. Mutz, Oleg Rashkovskiy, Joseph R. S. Molnar, Chris P. Dury, Andrew Laszlo, Rudy Ruano
  • Publication number: 20060264236
    Abstract: A method of processing information received in the form of a message from a mobile handheld device with a camera, where the message includes data representing an image of a document and an identifier of a type of said document. The method preferably provides image enhancement, information recognition and analysis selected based on the identified document type. An output of the system is preferably provided to the user in the form specified by the user, such as an e-mail.
    Type: Application
    Filed: May 18, 2005
    Publication date: November 23, 2006
    Applicant: Mobilescan, Inc.
    Inventors: Andrew Mutz, Oleg Rashkovskiy, Joseph Molnar, Chris Dury, Andrew Laszlo, Rudy Ruano
  • Patent number: 5366071
    Abstract: A combined workbench for a fly-fisherman is collapsible to form a storage and carry-case. The workbench includes a main body unit having storage compartments and removable cover panels for providing a work surface. A lid member providing storage compartments is attached along a back edge of the main unit. Wing tray units provide auxiliary storage areas on either side of the main body unit. A clamp assembly stowed within the main body is adapted for securing a fly-tying vise in selected operational portions. A leg compartment in the main body houses fold-out legs that are selectively deployable for supporting the workbench. A frame assembly pivotally attached within the leg compartment is swingably deployable for holding a waste disposal bag.
    Type: Grant
    Filed: February 15, 1994
    Date of Patent: November 22, 1994
    Inventor: Andrew Laszlo