Patents by Inventor Andrew M. Bradbury
Andrew M. Bradbury has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10954275Abstract: Modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, a modified Fc-binding domain includes or consists of the amino acid sequence of any one of SEQ ID NOs: 4, 6, or 8. Nucleic acids encoding the modified Fc-binding domains or polypeptides including one or more of the modified Fc-binding domains are also provided. In some embodiments, the nucleic acids are included in a vector and may be operably linked to a promoter. Methods for purifying a polypeptide including one or more Fc regions are also provided. Kits and reagents including the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are linked to a solid support.Type: GrantFiled: August 3, 2018Date of Patent: March 23, 2021Assignee: Triad National Security, LLCInventors: Ramesh Jha, Charlie Strauss, Andrew M. Bradbury
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Patent number: 9637528Abstract: The invention provides directed evolution methods for improving the folding, solubility and stability (including thermostability) characteristics of polypeptides. In one aspect, the invention provides a method for generating folding and stability-enhanced variants of proteins, including but not limited to fluorescent proteins, chromophoric proteins and enzymes. In another aspect, the invention provides methods for generating thermostable variants of a target protein or polypeptide via an internal destabilization baiting strategy. Internally destabilization a protein of interest is achieved by inserting a heterologous, folding-destabilizing sequence (folding interference domain) within DNA encoding the protein of interest, evolving the protein sequences adjacent to the heterologous insertion to overcome the destabilization (using any number of mutagenesis methods), thereby creating a library of variants. The variants in the library are expressed, and those with enhanced folding characteristics selected.Type: GrantFiled: October 2, 2008Date of Patent: May 2, 2017Assignee: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Csaba Kiss, Geoffrey S. Waldo
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Patent number: 8420794Abstract: Humanized recombinant and monoclonal antibodies specific for the ectodomain of the influenza virus M2 ion channel protein are disclosed. The antibodies of the invention have anti-viral activity and may be useful as anti-viral therapeutics and/or prophylactic/vaccine agents for inhibiting influenza virus replication and for treating individuals infected with influenza.Type: GrantFiled: November 15, 2011Date of Patent: April 16, 2013Assignee: Los Alamos National Security, LLCInventor: Andrew M. Bradbury
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Patent number: 8227242Abstract: The invention relates to a novel phagemid display system for packaging phagemid DNA into phagemid particles which completely avoids the use of helper phage. The system of the invention incorporates the use of bacterial packaging cell lines which have been transformed with helper plasmids containing all required phage proteins but not the packaging signals. The absence of packaging signals in these helper plasmids prevents their DNA from being packaged in the bacterial cell, which provides a number of significant advantages over the use of both standard and modified helper phage. Packaged phagemids expressing a protein or peptide of interest, in fusion with a phage coat protein such as g3p, are generated simply by transfecting phagemid into the packaging cell line.Type: GrantFiled: December 7, 2006Date of Patent: July 24, 2012Assignee: Los Alamos National Security, LLCInventor: Andrew M. Bradbury
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Patent number: 8168411Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: GrantFiled: November 29, 2011Date of Patent: May 1, 2012Assignee: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss
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Publication number: 20120077960Abstract: Binding ligands with intrinsic fluorescence (“fluorobodies”), fluorobody libraries, and methods of preparing fluorobodies are provided. In one aspect, the invention provides fluorobodies generated from a highly stable, artificial fluorescent protein, eCGP123 and derivatives thereof.Type: ApplicationFiled: March 12, 2010Publication date: March 29, 2012Applicant: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss, Devin Close
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Publication number: 20120077266Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: ApplicationFiled: November 29, 2011Publication date: March 29, 2012Applicant: LOS ALAMOS NATIONAL SECURITY, LLCInventors: ANDREW M. BRADBURY, GEOFFREY S. WALDO, CSABA KISS
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Publication number: 20120064085Abstract: Humanized recombinant and monoclonal antibodies specific for the ectodomain of the influenza virus M2 ion channel protein are disclosed. The antibodies of the invention have anti-viral activity and may be useful as anti-viral therapeutics and/or prophylactic/vaccine agents for inhibiting influenza virus replication and for treating individuals infected with influenza.Type: ApplicationFiled: November 15, 2011Publication date: March 15, 2012Inventor: Andrew M. Bradbury
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Patent number: 8080244Abstract: Humanized recombinant and monoclonal antibodies specific for the ectodomain of the influenza virus M2 ion channel protein are disclosed. The antibodies of the invention have anti-viral activity and may be useful as anti-viral therapeutics and/or prophylactic/vaccine agents for inhibiting influenza virus replication and for treating individuals infected with influenza.Type: GrantFiled: November 20, 2009Date of Patent: December 20, 2011Assignee: Los Alamos National Security, LLCInventor: Andrew M. Bradbury
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Patent number: 8067541Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: GrantFiled: February 8, 2011Date of Patent: November 29, 2011Assignee: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss
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Publication number: 20110245464Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: ApplicationFiled: February 8, 2011Publication date: October 6, 2011Applicant: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss
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Patent number: 7910700Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: GrantFiled: December 19, 2008Date of Patent: March 22, 2011Assignee: Los Alamos National Security, LLCInventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss
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Patent number: 7868152Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: GrantFiled: September 15, 2009Date of Patent: January 11, 2011Assignee: Los Alamos National Security, LLCInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Publication number: 20100222551Abstract: Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.Type: ApplicationFiled: December 19, 2008Publication date: September 2, 2010Inventors: Andrew M. Bradbury, Geoffrey S. Waldo, Csaba Kiss
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Publication number: 20100215662Abstract: Humanized recombinant and monoclonal antibodies specific for the ectodomain of the influenza virus M2 ion channel protein are disclosed. The antibodies of the invention have anti-viral activity and may be useful as anti-viral therapeutics and/or prophylactic/vaccine agents for inhibiting influenza virus replication and for treating individuals infected with influenza.Type: ApplicationFiled: November 20, 2009Publication date: August 26, 2010Inventor: Andrew M. Bradbury
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Publication number: 20100009441Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: ApplicationFiled: September 15, 2009Publication date: January 14, 2010Applicant: LOS ALAMOS NATIONAL LABORATORYInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Patent number: 7589182Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: GrantFiled: September 12, 2007Date of Patent: September 15, 2009Assignee: Los Alamos National Security, LLCInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Publication number: 20090142820Abstract: The invention provides directed evolution methods for improving the folding, solubility and stability (including thermostability) characteristics of polypeptides. In one aspect, the invention provides a method for generating folding and stability-enhanced variants of proteins, including but not limited to fluorescent proteins, chromophoric proteins and enzymes. In another aspect, the invention provides methods for generating thermostable variants of a target protein or polypeptide via an internal destabilization baiting strategy. Internally destabilization a protein of interest is achieved by inserting a heterologous, folding-destabilizing sequence (folding interference domain) within DNA encoding the protein of interest, evolving the protein sequences adjacent to the heterologous insertion to overcome the destabilization (using any number of mutagenesis methods), thereby creating a library of variants. The variants in the library are expressed, and those with enhanced folding characteristics selected.Type: ApplicationFiled: October 2, 2008Publication date: June 4, 2009Inventors: Andrew M. Bradbury, Csaba Kiss, Geoffrey S. Waldo
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Patent number: 7135310Abstract: The present invention provides methods of amplifying target sequences without including regions flanking the target sequence in the amplified product or imposing amplification primer sequences on the amplified product. Also provided are methods of preparing a library from such amplified target sequences.Type: GrantFiled: June 11, 2002Date of Patent: November 14, 2006Assignee: The Regents of the University of CaliforniaInventors: Andrew M. Bradbury, Ahmet Zeytun
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Publication number: 20030207282Abstract: The present invention provides methods of amplifying target sequences without including regions flanking the target sequence in the amplified product or imposing amplification primer sequences on the amplified product. Also provided are methods of preparing a library from such amplified target sequences.Type: ApplicationFiled: June 11, 2002Publication date: November 6, 2003Applicant: Los Alamos National LaboratoryInventors: Andrew M. Bradbury, Ahmet Zeytun