Patents by Inventor Andrew Main
Andrew Main has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240060075Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: ApplicationFiled: August 23, 2023Publication date: February 22, 2024Inventors: Andrew MAIN, Grzegorz WOJCIECHOWSKI, Yue YANG, Lishan ZHAO
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Patent number: 11767533Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: GrantFiled: July 9, 2021Date of Patent: September 26, 2023Assignee: AMYRIS, INC.Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
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Publication number: 20220251606Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.Type: ApplicationFiled: March 9, 2022Publication date: August 11, 2022Inventors: Kevin GEORGE, Andrew MAIN, Chia-Hong TSAI
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Patent number: 11293033Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.Type: GrantFiled: May 18, 2017Date of Patent: April 5, 2022Assignee: AMYRIS, INC.Inventors: Kevin George, Andrew Main, Chia-Hong Tsai
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Publication number: 20220002735Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: ApplicationFiled: July 9, 2021Publication date: January 6, 2022Inventors: Andrew MAIN, Grzegorz WOJCIECHOWSKI, Yue YANG, Lishan ZHAO
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Patent number: 11085046Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: GrantFiled: October 28, 2016Date of Patent: August 10, 2021Assignee: AMYRIS, INC.Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
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Publication number: 20200200942Abstract: A downhole logging tool includes a tool body housing an opto-electronics assembly. The opto-electronics assembly includes a circuit board and an opto-electronics module coupled to the circuit board and receiving control signals from the circuit board. The opto-electronics module includes a light source, a reference detector, a measurement detector, and a first beam splitter positioned with respect to the light source and the reference detector. The first beam splitter directs a first portion of light emitted from the light source into the reference detector and a second portion of the light emitted from the light source into an optical conduit. The opto-electronics module also includes a second beam splitter positioned with respect to the optical conduit and the measurement detector. The second beam splitter directs at least a portion of light received from the optical conduit into the measurement detector.Type: ApplicationFiled: December 19, 2019Publication date: June 25, 2020Applicant: Baker Hughes Oilfield Operations LLCInventors: Andrew Main, Stephen Day, Ian Davidson, James David Ratcliffe
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Patent number: 10653449Abstract: A surgical access device comprises a seal assembly (51) having a plurality of part circular sealing members (55) each attached to a common ring (53) which is held within the device about its longitudinal axis. In use, the sealing members are located radially inwards of the ring in a stacked relationship. When not within the device, the sealing members are movable from a position radially outwards of the ring to a position radially inwards of the ring. A seal core comprises the seal assembly and a protective member which, in use, is located on the proximal side of the seal assembly. A method of assembling the surgical access device is also provided.Type: GrantFiled: January 11, 2016Date of Patent: May 19, 2020Assignee: SURGICAL INNOVATIONS LIMITEDInventors: David Andrew Main, Christopher David Harrison
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Publication number: 20190382773Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nude is acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: ApplicationFiled: October 28, 2016Publication date: December 19, 2019Inventors: Andrew MAIN, Grzegorz WOJCIECHOWSKI, Yue YANG, Lishan ZHAO
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Publication number: 20190144887Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.Type: ApplicationFiled: May 18, 2017Publication date: May 16, 2019Inventors: Kevin GEORGE, Andrew MAIN, Chia-Hong TSAI
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Publication number: 20180338778Abstract: An obturator includes an elongate shaft extending between a proximal end and a distal end, the elongate shaft having an opening at the proximal end and defining an elongate cavity which is sized and shaped to receive an optical instrument. The obturator is provided with an axially extending finger for radial movement inwardly to engage the outer surface of the optical instrument located within the elongate cavity. The finger may be a latch member with a latch element and able to be moved between a first position, in which the latch element is located relatively radially inward, and a second position in which the latch element is located relatively radially outward. With the obturator located within a cannula body, an instrument inserted into the obturator causes the latch element of the latch member to engage with the cannula to prevent relative axial movement therebetween.Type: ApplicationFiled: December 22, 2016Publication date: November 29, 2018Applicant: Surgical Innovations LimitedInventors: David Andrew Main, Christopher David Harrison
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Publication number: 20180021063Abstract: A surgical access device comprises a seal assembly (51) having a plurality of part circular sealing members (55) each attached to a common ring (53) which is held within the device about its longitudinal axis. In use, the sealing members are located radially inwards of the ring in a stacked relationship. When not within the device, the sealing members are movable from a position radially outwards of the ring to a position radially inwards of the ring. A seal core comprises the seal assembly and a protective member which, in use, is located on the proximal side of the seal assembly. A method of assembling the surgical access device is also provided.Type: ApplicationFiled: January 11, 2016Publication date: January 25, 2018Applicant: SURGICAL INNOVATIONS LIMITEDInventors: David Andrew MAIN, Christopher David HARRISON
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Publication number: 20120270260Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: ApplicationFiled: July 5, 2012Publication date: October 25, 2012Applicant: AMYRIS, INC.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main
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Patent number: 8236512Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: GrantFiled: February 1, 2012Date of Patent: August 7, 2012Assignee: Amyris, Inc.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main
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Publication number: 20120196315Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: ApplicationFiled: February 1, 2012Publication date: August 2, 2012Applicant: AMYRIS, INC.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main