Patents by Inventor Andrew Mark Cigan
Andrew Mark Cigan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11560568Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.Type: GrantFiled: August 31, 2015Date of Patent: January 24, 2023Inventors: Andrew Mark Cigan, Huirong Gao, Zhan-Bin Liu, Jasdeep Mutti, Dean Podlich, Christopher Jay Scelonge
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Patent number: 11535850Abstract: The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.Type: GrantFiled: January 21, 2022Date of Patent: December 27, 2022Assignee: Genus PLCInventors: Andrew Mark Cigan, Jonathan Edward Lightner, Matthew Scott Culbertson, William Thomas Christianson, Benjamin Beaton, Brian Burger, Dylan Barnes, Matthew Campbell
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Publication number: 20220403411Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.Type: ApplicationFiled: June 2, 2022Publication date: December 22, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MARC C ALBERTSEN, ANDREW MARK CIGAN, HOWARD P HERSHEY, MICHAEL LASSNER, YONGZHONG WU
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Publication number: 20220356485Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 19, 2022Publication date: November 10, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
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Patent number: 11459576Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: July 10, 2020Date of Patent: October 4, 2022Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
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Patent number: 11377668Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.Type: GrantFiled: November 13, 2018Date of Patent: July 5, 2022Inventors: Marc C. Albertsen, Andrew Mark Cigan, Howard P. Hershey, Michael Lassner, Yongzhong Wu
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Patent number: 11371050Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: GrantFiled: May 12, 2016Date of Patent: June 28, 2022Inventors: Andrew Mark Cigan, Giedrius Gasiunas, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
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Publication number: 20220177900Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: February 22, 2022Publication date: June 9, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, PHILLIP A PATTEN, JOSHUA K YOUNG
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Publication number: 20220162628Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.Type: ApplicationFiled: September 2, 2021Publication date: May 26, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, MARK JACOB GADLAGE, HUIRONG GAO, ROBERT B MEELEY, JOSHUA K YOUNG
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Publication number: 20220135976Abstract: The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv).Type: ApplicationFiled: January 21, 2022Publication date: May 5, 2022Applicant: Genus PLCInventors: Andrew Mark Cigan, Jonathan Edward Lightner, Matthew Scott Culbertson, William Thomas Christianson, Benjamin Beaton, Brian Burger, Dylan Barnes, Matthew Campbell
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Publication number: 20220056439Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.Type: ApplicationFiled: November 11, 2021Publication date: February 24, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, TIM FOX, MANJIT SINGH
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Publication number: 20220042017Abstract: The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv).Type: ApplicationFiled: October 29, 2021Publication date: February 10, 2022Applicant: Genus PLCInventors: Andrew Mark Cigan, Jonathan Edward Lightner, Matthew Scott Culbertson, William Thomas Christianson, Benjamin Beaton, Brian Burger, Dylan Barnes, Matthew Campbell
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Patent number: 11208659Abstract: The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.Type: GrantFiled: May 4, 2021Date of Patent: December 28, 2021Assignee: Genus PLCInventors: Andrew Mark Cigan, Jonathan Edward Lightner, Matthew Scott Culbertson, William Thomas Christianson, Benjamin Beaton, Brian Burger, Dylan Barnes, Matthew Campbell
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Patent number: 11203752Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.Type: GrantFiled: December 10, 2018Date of Patent: December 21, 2021Inventor: Andrew Mark Cigan
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Publication number: 20210348165Abstract: The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv).Type: ApplicationFiled: May 4, 2021Publication date: November 11, 2021Applicant: Genus PLCInventors: Andrew Mark Cigan, Jonathan Edward Lightner, Matthew Scott Culbertson, William Thomas Christianson, Benjamin Beaton, Brian Burger, Dylan Barnes, Matthew Campbell
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Patent number: 11136589Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.Type: GrantFiled: January 25, 2017Date of Patent: October 5, 2021Inventors: Andrew Mark Cigan, Mark Jacob Gadlage, Huirong Gao, Robert B Meeley, Joshua K Young
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Publication number: 20210254096Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.Type: ApplicationFiled: April 29, 2021Publication date: August 19, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MARC C ALBERTSEN, UTE BAUMANN, ANDREW MARK CIGAN, MANJIT SINGH, ELISE TUCKER, RYAN WHITFORD
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Publication number: 20210222192Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.Type: ApplicationFiled: December 10, 2018Publication date: July 22, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: UTE BAUMANN, ANDREW MARK CIGAN, MARGARET ANNE PALLOTTA, MANJIT SINGH, RADOSLAW SUCHECKI, NATHAN SPENCER WATSON-HAIGH, RYAN WHITFORD
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Patent number: 11015209Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.Type: GrantFiled: September 21, 2015Date of Patent: May 25, 2021Inventors: Marc C Albertsen, Ute Baumann, Andrew Mark Cigan, Manjit Singh, Elise Tucker, Ryan Whitford
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Publication number: 20210079411Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid.Type: ApplicationFiled: September 23, 2020Publication date: March 18, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: ANDREW MARK CIGAN