Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.

Abstract: A therapeutic roller includes a cavity defined in an interior of the therapeutic roller. A first percussion device is disposed within the cavity. The percussion device includes a first reciprocating percussion head. A first aperture is defined in an exterior of the therapeutic roller. The first percussion head extends through the first aperture so as to contact a bodily region of a user. The first percussion device delivers percussion massage therapy to the bodily region of the user in conjunction with therapeutic roller therapy.

Abstract: A hose nozzle for spraying fluids is disclosed. The hose nozzle comprises a faceplate rotatable about a central pivot axis to two or more user-selectable rotational orientations. The faceplate comprises an upstream sealing surface and two or more entry openings. A seal member defines a seal opening that comprises a portion of a fluid flow pathway within the hose nozzle. The seal member may comprise a downstream sealing surface.

Abstract: Disclosed herein are compounds for treating a condition modulated by calcium channel ion activity and pharmaceutical compositions comprising the compounds, including the compounds comprising a piperazine core, a 1,4-diazepane core or a 1,5-diazepane core. Also disclosed herein are methods using the compounds to treat a disease or condition relating to aberrant function or activity of a T-type calcium channel.

Abstract: Structures for reducing forward loads in compressors are described. A compressor includes inner and outer circumferential support structures. The inner circumferential support structure includes an aft-most and forward spacer arms. The compressor also includes two stages, each including a vane having a root positioned at the outer circumferential support structure and a tip positioned radially inward from the root, and a rotor extending radially from the spacer arm adjacent to the vane. An intersection of the rotors and spacer arms defines centrally located points. An arrangement of a first line extending through the points forms an angle with a second line parallel to a longitudinal centerline and extending through the tips of the vanes. The angle is greater than 0° and less than or equal to 45°.

Abstract: Disclosed herein are compounds for treating a condition modulated by calcium channel ion activity and pharmaceutical compositions comprising the compounds, wherein the compounds comprise an azaspirononane core and left- and right-hand substitutions of the azaspirononane core. Also disclosed herein are methods using the compounds to treat a disease or condition relating to aberrant function or activity of a T-type calcium channel.

Abstract: Provided, in part, are compounds of Formula I. pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof, which are useful in the treatment of conditions associated with the activity of sodium channels. Methods of treating a disease or condition relating to aberrant function of a sodium ion channel including neurological disorders (e.g., Dravet syndrome, epilepsy), pain, and neuromuscular disorders are also provided herein.

Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Abstract: The present invention is directed to, in part, fused heteroaryl compounds and compositions useful for preventing and/or treating a disease or condition relating to aberrant function of a voltage-gated, sodium ion channel, for example, abnormal late/persistent sodium current. Methods of treating a disease or condition relating to aberrant function of a sodium ion channel including Dravet syndrome or epilepsy are also provided herein.

Abstract: A spreader with a material divider that is adjustable to change a stream of material which flows in an arbitrary manner to the centrifugal disk(s) to achieve a desired symmetrical or asymmetrical spread pattern.

Abstract: The present invention is directed to, in part, fused heteroaryl compounds and compositions useful for preventing and/or treating a disease or condition relating to aberrant function of a voltage-gated, sodium ion channel, for example, abnormal late/persistent sodium current. Methods of treating a disease or condition relating to aberrant function of a sodium ion channel including neurological disorders (e.g., Dravet syndrome, epilepsy), pain, and neuromuscular disorders are also provided herein.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: Provided, in part, are compounds of Formula I: pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof, which are useful in the treatment of conditions associated with the activity of sodium channels. Methods of treating a disease or condition relating to aberrant function of a sodium ion channel including neurological disorders (e.g., Dravet syndrome, epilepsy), pain, and neuromuscular disorders are also provided herein.

Abstract: The present disclosure relates generally to methods for improving traits or fitness in cattle, pigs, or aquatic organisms via gene editing. In particular, the methods comprise modifying genes in embryonic stem cells of livestock (e.g., cattle, pigs, or fish) for the purpose of improving a trait (e.g., promoting pathogen resistance, fertility, lactation, and native traits that support more rapid growth or feed efficiency). The methods particularly include CRISPR-CAS9 editing of porcine and bovine embryonic stem cells. Also disclosed herein are methods of producing and cultivating porcine embryonic stem cells under feeder free conditions.

Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

Abstract: Techniques are described for uniquely identifying a quadrant of a symmetrical vehicle, such as a symmetrical autonomous vehicle. The techniques include using service indicator lights to convey information regarding the status and operational mode of the vehicle to an observer and/or service technician. The service indicator lights may uniquely identify quadrants of the vehicle for orienting the vehicle with a representation of the vehicle to guide a technician to a particular location and component of the vehicle located within the identified quadrant.

Abstract: Mounting systems configured to mount vision systems such as goggles to target devices such as helmets include a gantry and carriage assembly having a hinge assembly with a pill and a tilt adjustment assembly that cooperates with the pill to move the carriage between various tilt positions and between a deploy and a stow position that is agnostic of deployed tilt setting. The tilt adjustment assembly includes a tilt member that moves in a front to back direction. The gantry and carriage assembly also includes a bore in an upper portion of the carriage and a stow insert that also cooperates with the pill to lock the pill into a stow detent and secure the gantry and carriage assembly in the stow position.

Abstract: A method for processing a control channel at a user agent (UA) to identify power control commands within a wireless communication system, the method comprising the steps of, at a user agent, monitoring for power control messages on a single carrier, decoding a message received on the single carrier to generate decoded information and using the decoded information to identify a power control command for each of at least first and second carriers.