Abstract: Non-human animals, e.g., mammals, e.g., mice or rats, are provided comprising an immunoglobulin heavy chain locus that comprises a rearranged human immunoglobulin heavy chain variable region nucleotide sequence. The rearranged human immunoglobulin heavy chain variable region nucleotide sequence may be operably linked to a heavy or light chain constant region nucleic acid sequence. Also described are genetically modified non-human animals comprising an immunoglobulin light chain locus comprising one or more but less than the wild type number of human immunoglobulin light chain variable region gene segments, which may be operably linked to a light chain constant region nucleic acid sequence. Also provided are methods for obtaining nucleic acid sequences that encode immunoglobulin light chain variable domains capable of binding an antigen in the absence of a heavy chain.

Abstract: A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire characterized by a limited number of light chain variable domains. Mice are provided that express just one or a few immunoglobulin light chain variable domains from a limited repertoire in their germline. Methods for making bispecific antibodies having universal light chains using mice as described herein, including human light chain variable regions, are provided. Methods for making human variable regions suitable for use in multispecific binding proteins, e.g., bispecific antibodies, and host cells are provided. Bispecific antibodies capable of binding first and second antigens are provided, wherein the first and second antigens are separate epitopes of a single protein or separate epitopes on two different proteins are provided.

Abstract: A modular building at a job site comprises one or more volumes templates, each comprising two or more core locations and a corridor all bounded within a volume footprint. A core for each core location is selected from a core catalog. Each core includes a base, at least three walls projecting upward from a floor of the base, a ceiling closing off an interior space, and an interior kit of internal walls, appliances, fixtures, and the like. Each volume template, the selected cores, and internal kits are constructed concurrently at a manufacturing facility along with external walls to complete each volume, which is transported to the job site. Adjacent volumes are connected together, with service lines of each core being connected with supply lines at the job site.

Abstract: A system is provided that receives a first message from a patient device containing patient information comprising at least one of demographic, health insurance, medication, and medical history and requesting an appointment with a first health care provider. If the first message does not designate a provider for the requested appointment, the system lists providers for review and selection. The system determines that the first provider one of designated and selected is a subscriber to automated check-in services provided by the system. The system verifies patient health insurance coverage and determines patient deductible balance and insurer copayment requirement, contacts the first provider, provides the patient information, and requests available appointment dates and times and patient account information.

Abstract: A system is provided that receives a first message from a patient device containing patient information comprising at least one of demographic, health insurance, medication, and medical history and requesting an appointment with a first health care provider. If the first message does not designate a provider for the requested appointment, the system lists providers for review and selection. The system determines that the first provider one of designated and selected is a subscriber to automated check-in services provided by the system. The system verifies patient health insurance coverage and determines patient deductible balance and insurer copayment requirement, contacts the first provider, provides the patient information, and requests available appointment dates and times and patient account information.