Abstract: The present disclosure relates to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associate protein 9 (Cas9) systems, and methods of use thereof for gene editing.

Abstract: Systems and methods for detecting single nucleotide polymorphisms (SNPs) associated with keratoconus (KC) in a sample from a subject are described.

Abstract: The present disclosure relates to single guide RNA (sgRNA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associate protein 9 (Cas9) system, and methods of use thereof for preventing, ameliorating or treating corneal dystrophies.

Abstract: Methods and compositions for the treatment of a corneal dystrophy in a subject in need thereof are provided. In one aspect, the method includes the step of obtaining a plurality of stem cells comprising a nucleic acid mutation in a corneal dystrophy target nucleic acid from the subject and manipulating the nucleic acid mutation in one or more stem cells of the plurality of stem cells to correct the nucleic acid mutation, thereby forming one or more manipulated stem cells. The manipulated stem cells are isolated and then transplanted into the subject. In some embodiments, the nucleic acid mutation is manipulated using CRISPR system.

Abstract: A method for fingerprinting an unknown music sample is disclosed. A plurality of known tracks may be segmented into reference samples. A reference fingerprint including a plurality of codes may be generated for each reference sample. An inverted index including, for each possible code value, a list of reference samples having reference fingerprints that contain the respective code value may be generated. An unknown fingerprint including a plurality of codes may be generated from the unknown music sample. A code match histogram may list candidate reference samples and associated scores, each score indicating a number of codes from the unknown fingerprint that match codes in the reference fingerprint. Time difference histograms may be generated for two or more reference samples having the highest scores. A determination may be made whether or not a single reference sample matches the unknown music sample based on a comparison of the time difference histograms.

Abstract: A method for fingerprinting an unknown music sample is disclosed. A plurality of known tracks may be segmented into reference samples. A reference fingerprint including a plurality of codes may be generated for each reference sample. An inverted index including, for each possible code value, a list of reference samples having reference fingerprints that contain the respective code value may be generated. An unknown fingerprint including a plurality of codes may be generated from the unknown music sample. A code match histogram may list candidate reference samples and associated scores, each score indicating a number of codes from the unknown fingerprint that match codes in the reference fingerprint. Time difference histograms may be generated for two or more reference samples having the highest scores. A determination may be made whether or not a single reference sample matches the unknown music sample based on a comparison of the time difference histograms.