Abstract: Conventional methods for the isolation and identification of specific RNA-protein complexes are plagued by a number of problems not encountered in genomics or proteomics. Here we describe a two step affinity purification method used to isolate RNA-protein complexes. The TRAP (Tandem RNA Affinity Purification) tag is a dual RNA tagging system that facilitates gentle purification of RNA molecules along with the proteins, RNAs and other small molecules specifically associated with them.

Abstract: In a telecommunication system, a fault supervision and management system includes a chain system of diagnose and inference objects connected one after another in a fault propagation direction. The locations of these objects are determined so as to enable them to supervise, in a supervision domain, one phenomenon each, which may be caused by faults in the telecommunication system. The objects are further located so as to enable them to communicate with, affect, and interact with each other in case of the emergence of a fault, for localizing faults in the telecommunication system. Each diagnose and inference object uses one or more measurement point objects for observing the emergence of the phenomenon supervised by the diagnose and inference object and for reporting to the diagnose and inference object. Several measurement point objects may be grouped in a measurement combinatory object which puts together and processes data from measurement point objects.