Abstract: Devices, kits, and methods for heating a drug formulation in an injection device are disclosed. An embodiment of the device includes a housing enclosure having a first housing shell and a second housing shell, and a plurality of bladders adjacent to an inner surface of the first housing shell and adjacent to an inner surface of the second housing shell. The housing enclosure is designed to receive the injection device. When an edge of the first housing shell and an edge of the second housing shell couple, the bladders contact a portion of the injection device and heat the drug formulation in the injection device. An embodiment of the kit includes this device and the injection device.

Abstract: A serum-free culture media for expansion of the number of cells and related method of use are disclosed. The disclosed media can be for use in cultured food applications. The disclosed media can include a baseline serum-free and animal component-free culture media and a plant protein composition. The plant protein composition can comprise unhydrolyzed plant proteins. The plant protein composition can comprise plant proteins that are 3 kDa in size or greater. The plant protein composition can be present in the serum-free cell culture media at a final concentration of 0.05 g/L to 1 g/L. The baseline media, in use, provides a baseline growth capability for expansion of the numbers of muscle satellite cells for use in cultured food applications. The disclosed media, in use, provides an improved growth capability for expansion of the number of muscle satellite cells. Methods of making and using the disclosed media are also disclosed.

Abstract: The present disclosure relates to cultured tissue, methods for production of self-sufficient modified cells for producing cultured tissue. Further, the disclosure provides methods of generating cultured meat products without exogenous addition of growth hormones.

Abstract: A serum-free and animal-component-free culture media for expansion of muscle satellite cells is disclosed. The disclosed media can be for use in cultured food applications. The disclosed media can include a baseline serum-free and animal component-free culture media and a recombinant version of albumin. The baseline media, in use, provides a baseline growth capability for expansion of the muscle satellite cells for use in cultured food applications. The disclosed media, in use, provides an improved growth capability for expansion of the muscle satellite cells. The improved growth capability is at least 50% greater than the baseline growth capability. Methods of making and using the disclosed media are also disclosed.

Abstract: The present disclosure relates to cultured adipose tissue. In one embodiment, the cultured adipose tissue is produced by culturing adipose cells in a culture media in vitro, harvesting the adipose cells after a desired amount of adipose cells are produced, and aggregating the harvested adipose cells to provide the cultured adipose tissue. In some embodiments, aggregating the harvested adipose cells comprises mixing the harvested adipose cells with a hydrogel or binder in a three-dimensional (3D) mold. In other embodiments, aggregating the harvested adipose cells comprises cross-linking the harvested adipose cells in a 3D mold. The cultured adipose tissue have a defined 3D shape and a size on the macroscale. In some embodiments, the cultured adipose tissue may be a food product.

Abstract: The present invention provides animal cells that have been engineered to express one or more viral antigens. The engineered cells can be formulated into cultured meat products that can be used as edible vaccines.

Abstract: Provided herein are genetically engineered mammalian cells that endogenously express one or more phytochemicals, vitamins, or therapeutic agents and suitable for use in a cultured meat product. Methods of making and using the genetically engineered mammalian cells and the cultured meat products are also provided.