Abstract: Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided.

Abstract: Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided.

Abstract: A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

Abstract: Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

Abstract: A process for purifying a liquid feedstock comprising a monoclonal antibody and impurities, the process comprising passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified monoclonal antibody and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v) which include a multiple inlet flow-controller comprising two or more variable flow inlet valves for in situ production of a bioprocessing liquid by combining at least two liquids in a desired ratio. One of the units performs chromatography and another performs viral inactivation. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

Abstract: Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided.

Abstract: Implementations disclose methods and systems for rendering, by a browser, a content item projected on a mesh. A method includes providing, by a browser executing on a user device, an application programming interface (API) to communicate with a web application that includes a user interface to present a content item on the user device; receiving, by the browser from the web application via the API, an instruction to project the content item on a mesh, where the instruction identifies the content item and the mesh; rendering, by the browser and without involvement of the web application, a first frame of the content item projected on the mesh in a first orientation; and causing, by the browser, the rendered first frame to be displayed on the user device.

Abstract: Implementations disclose methods and systems for rendering, by a browser, a content item projected on a mesh. A method includes providing, by a browser executing on a user device, an application programming interface (API) to communicate with a web application that includes a user interface to present a content item on the user device; receiving, by the browser from the web application via the API, an instruction to project the content item on a mesh, where the instruction identifies the content item and the mesh; rendering, by the browser and without involvement of the web application, a first frame of the content item projected on the mesh in a first orientation; and causing, by the browser, the rendered first frame to be displayed on the user device.

Abstract: A method, apparatus and computer program product are hereby provided to detect anatomical elements in a medical image. In this regard, the method, apparatus, and computer program product may receive a test image and generate a classified image by applying an image classifier to the test image. The image classifier may include at least one decision tree for evaluating at least one pixel value of the test image and the classified image may include a plurality of pixel values. Each pixel value may be associated with a probability that an anatomical element is located at the pixel location. The method, apparatus, and computer program product may also evaluate the classified image using an anatomical model to detect at least one anatomical element within the classified image.

Abstract: Methods and systems for interactively segmenting 3D image data are provided. An initial segmentation of the 3D image data is obtained, and for each of a plurality of image regions, a segmentation uncertainty indicator for the initial image segmentation is associated with the image region and a strength is assigned to the segmentation uncertainty indicator. A low-confidence region in the 3D image data is identified based at least in part on proximity of the low-confidence region to the image regions and strengths of the corresponding segmentation uncertainty indicators. An optimization routine may be applied to an objective function whose value depends at least in part on proximity of the candidate region to the image regions and the strengths of the corresponding uncertainty indicators to identify the low-confidence region from among a plurality of candidate regions.

Abstract: A method, apparatus and computer program product are hereby provided to detect anatomical elements in a medical image. In this regard, the method, apparatus, and computer program product may receive a test image and generate a classified image by applying an image classifier to the test image. The image classifier may include at least one decision tree for evaluating at least one pixel value of the test image and the classified image may include a plurality of pixel values. Each pixel value may be associated with a probability that an anatomical element is located at the pixel location. The method, apparatus, and computer program product may also evaluate the classified image using an anatomical model to detect at least one anatomical element within the classified image.

Abstract: Methods and systems for interactively segmenting 3D image data are provided. An initial segmentation of the 3D image data is obtained, and for each of a plurality of image regions, a segmentation uncertainty indicator for the initial image segmentation is associated with the image region and a strength is assigned to the segmentation uncertainty indicator. A low-confidence region in the 3D image data is identified based at least in part on proximity of the low-confidence region to the image regions and strengths of the corresponding segmentation uncertainty indicators. An optimization routine may be applied to an objective function whose value depends at least in part on proximity of the candidate region to the image regions and the strengths of the corresponding uncertainty indicators to identify the low-confidence region from among a plurality of candidate regions.

Abstract: The present invention provides improved methods for the purification of factor XIII. In particular, the methods provide compositions containing 5% or less contaminating proteins. In particular embodiments of the present invention the methods provide purified factor XIII compositions comprising less than 1% activated factor XIII, less than 2% protein aggregates, and/or less than 5% charge isomers of factor XIII. The methods do not require the use a precipitation or crystallization step common to prior methods of isolating factor XIII. Instead, the method uses immobilized metal affinity chromatography to remove various contaminants common to recombinant expression of factor XIII. Further, a combination of various chromatography methods including ion exchange chromatography, hydrophobic affinity chromatography, and immobilized metal affinity chromatography comprise a simple and less expensive method to produce a pharmaceutical grade factor XIII product at high yield.

Abstract: This invention relates to methods for use in industrial production of recombinant globular Adiponectin (gAdiponectin). Specifically, the present invention provides a purification process suitable for production of high amounts of pure gAdiponectin. gAdiponectin is expressed in E. coli, and a purification method with which about 30 grams of gAdiponectin could be obtained from 100 liters of cell culture has been set up.

Abstract: The present invention provides improved methods for the purification of factor XIII. In particular, the methods provide compositions containing 5% or less contaminating proteins. In particular embodiments of the present invention the methods provide purified factor XIII compositions comprising less than 1% activated factor XIII, less than 2% protein aggregates, and/or less than 5% charge isomers of factor XIII. The methods do not require the use a precipitation or crystallization step common to prior methods of isolating factor XIII. Instead, the method uses immobilized metal affinity chromatography to remove various contaminants common to recombinant expression of factor XIII. Further, a combination of various chromatography methods including ion exchange chromatography, hydrophobic affinity chromatography, and immobilized metal affinity chromatography comprise a simple and less expensive method to produce a pharmaceutical grade factor XIII product at high yield.