Patents by Inventor Andrew TRANS
Andrew TRANS has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240209432Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.Type: ApplicationFiled: June 28, 2023Publication date: June 27, 2024Applicant: Roche Sequencing Solutions, Inc.Inventors: Frank BERGMANN, Christoph SEIDEL, Andrew TRANS, Dmitriy GREMYACHINSKIY, Hannes KUCHELMEISTER, Lars HILLRINGHAUS
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Publication number: 20230416813Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: June 19, 2023Publication date: December 28, 2023Applicant: Roche Sequencing Solutions, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Patent number: 11739378Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.Type: GrantFiled: March 15, 2021Date of Patent: August 29, 2023Assignees: Roche Sequencing Solutions, Inc., Roche Molecular Systems, Inc.Inventors: Frank Bergmann, Christoph Seidel, Andrew Trans, Dmitriy Gremyachinskiy, Hannes Kuchelmeister, Lars Hillringhaus
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Patent number: 11725236Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: April 27, 2021Date of Patent: August 15, 2023Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20220090189Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.Type: ApplicationFiled: March 15, 2021Publication date: March 24, 2022Applicant: Roche Sequencing Solutions, Inc.Inventors: Frank BERGMANN, Christoph SEIDEL, Andrew TRANS, Dmitriy GREMYACHINSKIY, Hannes KUCHELMEISTER, Lars HILLRINGHAUS
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Publication number: 20210324462Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: April 27, 2021Publication date: October 21, 2021Applicant: Roche Sequencing Solutions, Inc.Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Publication number: 20210317521Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.Type: ApplicationFiled: April 13, 2021Publication date: October 14, 2021Applicant: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Peter Crisalli, Andrew Trans, Ashwini Bhat
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Patent number: 11021745Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: February 11, 2019Date of Patent: June 1, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Patent number: 11008613Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.Type: GrantFiled: March 20, 2020Date of Patent: May 18, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Peter Crisalli, Andrew Trans, Ashwini Bhat
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Patent number: 10975426Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.Type: GrantFiled: August 26, 2016Date of Patent: April 13, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Frank Bergmann, Christoph Seidel, Andrew Trans, Dmitriy Gremyachinskiy, Hannes Kuchelmeister, Lars Hillringhaus
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Publication number: 20210088511Abstract: Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.Type: ApplicationFiled: September 30, 2020Publication date: March 25, 2021Applicant: Roche Diagnostics Operations, Inc.Inventors: Peter CRISALLI, Dmitriy GREMYACHINSKIY, Dieter HEINDL, Hannes KUCHELMEISTER, Michael SCHRAEML, Andrew TRANS
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Publication number: 20200216894Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.Type: ApplicationFiled: March 20, 2020Publication date: July 9, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Peter Crisalli, Andrew Trans, Ashwini Bhat
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Patent number: 10669580Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.Type: GrantFiled: August 23, 2017Date of Patent: June 2, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Peter Crisalli, Andrew Trans, Ashwini Bhat
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Publication number: 20190185927Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: February 11, 2019Publication date: June 20, 2019Applicant: Genia Technologies, Inc.Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Patent number: 10273536Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.Type: GrantFiled: March 10, 2016Date of Patent: April 30, 2019Assignees: GENIA TECHNOLOGIES, INC., ROCHE MOLECULAR SYSTEMS, INC.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech, Hui Tian, Robert Yuan, John Foster, Roger Chen
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Patent number: 10246743Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: December 20, 2016Date of Patent: April 2, 2019Assignee: GENIA TECHNOLOGIES, INC.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech
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Publication number: 20190002968Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.Type: ApplicationFiled: August 26, 2016Publication date: January 3, 2019Inventors: Frank BERGMANN, Christoph SEIDEL, Andrew TRANS, Dmitriy GREMYACHINSKIY, Hannes KUCHELMEISTER, Lars HILLRINGHAUS
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Publication number: 20180057870Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.Type: ApplicationFiled: August 23, 2017Publication date: March 1, 2018Inventors: Dmitriy Gremyachinskiy, Peter J. Crisalli, Andrew Trans, Ashwini Bhat
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Publication number: 20170198343Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: ApplicationFiled: December 20, 2016Publication date: July 13, 2017Inventors: Randall W. DAVIS, Edward Shian LIU, Eric Takeshi HARADA, Anne AGUIRRE, Andrew TRANS, James POLLARD, Cynthia CECH
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Patent number: 9567630Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.Type: GrantFiled: October 22, 2014Date of Patent: February 14, 2017Assignee: Genia Technologies, Inc.Inventors: Randall W. Davis, Edward Shian Liu, Eric Takeshi Harada, Anne Aguirre, Andrew Trans, James Pollard, Cynthia Cech