Abstract: Provided are systems and methods for tagging biomolecules of interest, methods of generating tagged biomolecules, and methods of use thereof. An adapter system comprises one or more epitope tags operably linked to an antibody binding domain (AbBD), which may be a photoreactive antibody binding domain (pAbBD). A pAbBD may be used to covalently photocrosslink epitope tags to immunoglobulins or immunoglobulin fragments, which may in turn be used to label biomolecules of interest. Such epitope-tagged immunoglobulins may be used in various biological research applications, including multiplexed staining assays and tandem affinity pulldown assays.

Abstract: This invention relates to conjugate antibody, drug and nanoparticle compositions and methods of generating the same. This invention further relates to methods of using same for imaging, diagnosing or treating a disease.

Abstract: The invention relates to the use of precursor peptides and B1 proteins from lasso peptide biosynthesis systems for affinity purification, proximity-based sortase-mediated protein purification and ligation, and detection of fusion proteins. For proximity-based sortase-mediated protein purification and ligation, the invention relates to techniques that link protein purification with conjugation to other agents, including therapeutic agents, imaging agents, or linkers.

Abstract: The invention relates to proximity-based sortase-mediated protein purification and ligation. Specifically, the invention relates to techniques that links protein expression/purification with conjugation to therapeutic agents, imaging agents, or linkers.

Abstract: Provided are compositions comprising phthalocyanine (PC)-loaded or naphthalocyanine (NC) dye-loaded nanoparticulate polymeric or lipid micelles and methods for visualization of tumors without optical imaging equipment, the methods comprising injecting a subject with the phthalocyanine (PC)-loaded nanoparticulate amphiphilic polymer or lipid micelles and b) detecting by endoscopy an accumulation of blue dye in a tumor and a demarcation of tumor margins. Also provided are methods for treating a tumor, the method comprising a) administering intravenously to a subject phthalocyanine (PC) dye-loaded or naphthalocyanine (NC) dye-loaded nanoparticulate amphiphilic polymer or lipid micelles; b) detecting by intraoperative photoacoustic imaging an accumulation of dye in a tumor and a demarcation of tumor margins; and/or c) irradiating the PC dye or the NC dye within the tumor with a laser to heat the tumor, thereby decreasing tumor cell viability and/or killing the tumor cells.

Abstract: A bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody comprising a cytotoxic lymphocyte or macrophage targeting domain and an autoantibody. Methods for producing bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody comprising a cytotoxic lymphocyte or macrophage targeting domain and an autoantibody. Methods for treating a subject in need thereof comprising administering to the subject an isolated bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody.

Abstract: The present disclosure provides compositions and methods for site-specific labeling of antibodies by proximity-based sortase-mediated ligation. The ligation method utilizes a non-canonical isopeptide ligation reaction catalyzed by newly identified variants of S. aureus sortase A. An antibody binding domain (e.g., protein A or protein G) is fused to the variants of SrtA to bring the enzyme into close proximity of an antibody, thereby significantly increases the efficiency of isopeptide bond formation.

Abstract: This invention relates to the compositions and methods for labeling antibodies and other proteins and targeting agents with a helical bundle protein that is functionalized with cargo. Cargos can include but are not limited to fluorescent dyes, haptens (e.g. biotin), contrast agents (e.g. gadolinium, radionuclides), chelated metals, therapeutic agents, sensitizers, or other small molecules. Specifically, provided herein are compositions having a helical bundle, that has been labeled at precisely defined locations with cargo, and that can be conjugated, attached or fused to an antibody or other targeting agent.

Abstract: A bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody comprising a cytotoxic lymphocyte or macrophage targeting domain and an autoantibody. Methods for producing bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody comprising a cytotoxic lymphocyte or macrophage targeting domain and an autoantibody. Methods for treating a subject in need thereof comprising administering to the subject an isolated bispecific cytotoxic lymphocyte or macrophage-redirecting autoantibody.

Abstract: Therapeutic compositions, comprising: an anti-reactive oxygen species agent and a pervious polymersome, the pervious polymersome encapsulating the anti-reactive oxygen species agent, and the pervious polymersome having therein channels defined by a channel diblock copolymer, the channels being arranged so as to retain at least some of the anti-reactive oxygen species agent within the pervious polymersome while allowing reactive oxygen species to pass into the pervious polymersome.

Abstract: The invention provides methods for stimulating a universal immune receptor-mediated response in a mammal using cells engineered to express a universal immune receptor that comprises an adaptor molecule, such as a SpyCatcher or a SpyTag moiety, a transmembrane domain, and an intracellular domain for T cell activation.

Abstract: The invention relates to antibody conjugates (e.g., a bispecific antibody), drug and nanoparticle compositions and methods and compositions for generating them. This invention further relates to methods of using these compositions for imaging, diagnosing or treating a disease.

Abstract: The invention provides compositions and methods for the cytoplasmic delivery of antibodies and other proteins. Specifically, provided herein are compositions having an anionic polypeptide and a cationic transfection agent for facilitating the cytoplasmic delivery of an antibody or a protein.

Abstract: The invention relates to antibody conjugates (e.g., a bispecific antibody), drug and nanoparticle compositions and methods and compositions for generating them. This invention further relates to methods of using these compositions to image, diagnose or treat a disease.

Abstract: The invention relates to amphiphilic dye-coated inorganic nanoparticle clusters and uses thereof. Specifically, the invention relates to cyanine and/or cyclic tetrapyrrole dye-coated metallic nanoparticle clusters for use in medical imaging and treatments.

Abstract: The invention provides compositions and methods for adoptive T cell therapy in treating a variety of disorders including cancer, infections, and autoimmune disorders. In one embodiment, the invention provides a universal immune receptor that comprises a protein or peptide tag, such as a SpyCatcher or a SpyTag moiety, bound to an extracellular hinge region, a transmembrane domain, and an intracellular domain for T cell activation.

Abstract: An ultrasonication apparatus is provided for improving catheter function. The ultrasonication apparatus includes a transducer configured to produce ultrasonic waves for ultrasonication and a chamber for containing a fluid having a passage for receiving a catheter. The chamber is adapted to receive the ultrasonic waves from the transducer.

Abstract: Contrast agents for x-ray imaging including stabilized metal nanoparticles and encapsulated nanoparticles, as well as methods for imaging tissue with these agents, are disclosed. Also disclosed are methods of dual energy x-ray imaging using metal nanoparticle contrast agents or encapsulated metal nanoparticles.

Abstract: The invention relates to proximity-based sortase-mediated protein purification and ligation. Specifically, the invention relates to techniques that links protein expression/purification with conjugation to therapeutic agents, imaging agents, or linkers.

Abstract: This invention relates to to conjugate antibody, drug and nanoparticle compositions and methods of generating the same. This invention further relates to methods of using same for imaging, diagnosing or treating a disease.