Abstract: Provided herein are compositions comprising a non-naturally occurring DNA binding protein that modulates expression of an endogenous gene. Provided herein are methods of use thereof of the compositions which comprise a non-naturally occurring DNA binding protein that modulates expression of an endogenous gene.

Abstract: A replacement heart valve system may include a replacement heart valve implant including an expandable framework and a plurality of valve leaflets disposed within the expandable framework, and a filter element secured to the expandable framework and configured to be deployed downstream of the expandable framework, the filter element having a closed downstream end. A replacement heart valve system may include a filter element configured to be deployed across a native heart valve, wherein the filter element includes a lumen extending therethrough, and a replacement heart valve implant configured to be deployed within the lumen of the filter element. A method of treating a native heart valve may include deploying a filter element across the native heart valve, expanding an expandable balloon within the lumen of the filter element, and thereafter, deploying a replacement heart valve implant within the lumen of the filter element.

Abstract: Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

Abstract: Methods of making an enhanced bioactive PRP implant include supplying a centrifuge container with a collagen-based scaffold pre-inserted onto a moveable filter within the vial. A centrifuge process for creating the PRP implant is followed, resulting in a PRP/collagen implant combination. Other biologics may be added to the container instead of or in addition to the collagen-based scaffold. Additionally, methods of fixing the PRP implant into place at the point of a tear or wound include applying the PRP implant adjacent to or within a meniscal tear, or underneath or on top of a rotator cuff tear. Suture or staples may then be routed either through or over the PRP implant to hold the implant in place.

Abstract: A transport vehicle, such as a vision guided vehicle, can comprise a drive portion constructed to facilitate movement of the transport vehicle and a load portion constructed to engage an object of interest. The load portion can comprise an object engagement apparatus and at least one sensor coupled to or disposed within a distal end of the object engagement apparatus, wherein the sensor can be at least a 2D sensor. The engagement apparatus can comprise forks, at least one fork having sensor coupled to or disposed within a fork tip. The 2D sensor can comprise a scanning LIDAR sensor arranged to collect information to identify a pickable pallet, for example.

Abstract: Methods and devices for vector-based targeting of the human central thalamus (CT) to guide deep brain stimulation (DBS) are disclosed. In some examples, electrode(s) each with a plurality contacts are provided. A three-dimensional orientation of a dominant axis of a central lateral nucleus dorsal tegmental tract medial component (CL/DTTm) fiber bundle of a human subject is determined. The contacts of the electrode(s) are positioned in the subject's CT fibers in substantial alignment with the three-dimensional orientation. An electrical stimulus is applied to the contacts to selectively activate the CT fibers. The positioning and the applying are carried out to maximize activation of a central lateral nucleus and medial dorsal tegmental tract fiber pathway in the subject and to minimize activation of a centromedian-parafascicularis fiber pathway in the subject. Methods and devices for surgical planning involving for vector-based targeting of the human CT to guide DBS are also disclosed.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for training a neural network to perform any one or more of a variety of machine learning tasks. For example, the neural network can be configured as a generative neural network, e.g., an autoregressive generative neural network.

Abstract: A hand-gesture tracking engine includes a first artificial intelligence (AI) model component configured and trained to automatically identify a hand-gesture made by a source player within a video game. A hand-gesture intent engine includes a second AI model component configured and trained to automatically determine a message for communication to a target player within the video game as conveyed by the hand-gesture identified by the hand-gesture tracking engine. A hand-gesture translation engine having a third AI model component configured and trained to automatically generate a communication to the target player that conveys the message as determined by the hand-gesture intent engine. The third AI model component is configured and trained to automatically control sending of the communication to the target player.

Abstract: Provided herein are compositions and methods for driving high expression of a transgene. Compositions and methods for driving high expression of a transgene comprising one or more human-derived regulatory elements, which, when operably linked to a transgene, can result in high expression of the transgene in one or more cell types or tissues.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for training a neural network to perform any one or more of a variety of machine learning tasks. For example, the neural network can be configured as a generative neural network, e.g., an autoregressive generative neural network.

Abstract: Systems and methods for enabling communication between users are described. One of the methods includes receiving, via a computer network, data regarding a plurality of real gestures made by a first user via a first user account. The data regarding the plurality of real gestures is received from a client device. The method further includes determining, from the data, whether a speed of occurrence of the plurality of real gestures is greater than a predetermined speed. After determining that the speed of occurrence of the plurality of real gestures is greater than the predetermined speed, the method includes determining one or more meanings of a combination of the plurality of real gestures. The method includes communicating the one or more meanings of the combination via a virtual avatar, controlled by the first user via the first user account, to a second user.

Abstract: A system may include an energy provision system, a first converter, a second converter, a traction system, and an auxiliary system. The energy provision system may provide a first voltage. The first converter may increase the first voltage from the energy provision system from the first voltage into at least a second voltage. The traction system of a vehicle may be powered by the second voltage from the first converter. The second converter may decrease the first voltage from the energy provision system from the first voltage to a third voltage which may be less than the second voltage. The auxiliary system of the vehicle may be powered by the third voltage from the second converter that may be less than the second voltage.

Abstract: Gaze tracking data representing a user's gaze is analyzed to determine one or more regions of interest. One or more gaze tracking parameters are determined from the gaze tracking data. Adjusted foveation data is determined representing an adjusted size and/or shape of one or more regions of interest in one or more images to be subsequently presented to the user based on the one or more gaze tracking parameters. The compression of the one or more transmitted images is adjusted so that fewer bits are needed to transmit data for portions of an image outside the one or more regions of interest than for portions of the image within the one or more regions of interest. Adjusting compression includes eliminating the region(s) of interest from images that are presented to the user during the saccade or blink.

Abstract: A method of targeting, which involves capturing a first video of a scene about a potential targeting coordinate by a first video sensor on a first aircraft; transmitting the first video and associated potential targeting coordinate by the first aircraft; receiving the first video on a first display in communication with a processor, the processor also receiving the potential targeting coordinate; selecting the potential targeting coordinate to be an actual targeting coordinate for a second aircraft in response to viewing the first video on the first display; and guiding a second aircraft toward the actual targeting coordinate; where positive identification of a target corresponding to the actual targeting coordinate is maintained from selection of the actual targeting coordinate.

Abstract: Provided herein are methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers comprising an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

Abstract: Provided herein are methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers comprising an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

Abstract: Provided herein are compositions and methods for driving high expression of a transgene. Compositions and methods for driving high expression of a transgene comprising one or more human-derived regulatory elements, which, when operably linked to a transgene, can result in high expression of the transgene in one or more cell types or tissues.

Abstract: A paver assembly includes a paving unit assembly having a first side wall, a second side wall, and an expandable frame connecting the first side wall and the second side wall together. The expandable frame can move the first side wall and the second side wall towards and away from one another to contract and expand the paving unit assembly so that a width of the paving unit assembly can be the same as a width of a surface paved by the paver assembly. The paver assembly can also include a first paving unit fixedly connected with respect to the first side wall and a second paving unit fixedly connected with respect to the second side wall, where the first paving unit and the second paving unit are offset with respect to one another and move past one another as the paver assembly expands and contracts.

Abstract: A new and innovative medical sizing instrument and method are provided for determining an appropriate depth and/or length of a clavicle hook plate for a patient. The provided medical sizing instrument may enable a surgeon to determine an appropriate hook depth without having to switch between different components, such as a series of templates. In an example, a surgeon may insert the medical sizing instrument into a patient the same as the surgeon would a clavicle hook plate, adjust the hook depth on the medical sizing instrument until a hook depth is obtained that appropriately maintains the anatomical alignment of the patient's clavicle and acromion, and read an indicated depth on the medical sizing instrument. The surgeon may then remove the medical sizing instrument from the patient and select the appropriate clavicle hook plate to insert into the patient.

Abstract: Provided herein are compositions and methods of use thereof comprising a non-naturally occurring DNA binding protein that modulates expression of an endogenous gene.