Patents by Inventor Andrew Zayac
Andrew Zayac has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11951478Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: GrantFiled: February 12, 2021Date of Patent: April 9, 2024Assignee: Combinati IncorporatedInventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Patent number: 11697844Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.Type: GrantFiled: October 30, 2019Date of Patent: July 11, 2023Assignee: Combinati IncorporatedInventors: Ju-Sung Hung, Megan Dueck, Andrew Zayac
-
Publication number: 20220243254Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.Type: ApplicationFiled: January 19, 2022Publication date: August 4, 2022Inventors: Ju-Sung Hung, Megan Dueck, Andrew Zayac
-
Publication number: 20220097054Abstract: The present disclosure provides systems, methods, and devices for processing a biological sample. The device may be a microfluidic device comprising a first channel, at least one chamber, and a second channel. The first channel may be in fluid communication with the chamber. The chamber may be configured to receive a portion of the biological sample from the channel. The second channel may be configured for pressurized outgassing of the chamber, first channel, or both the chamber and first channel.Type: ApplicationFiled: September 28, 2021Publication date: March 31, 2022Inventors: Ju-Sung HUNG, Felicia LINN, Andrew ZAYAC
-
Patent number: 11285478Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: GrantFiled: October 13, 2017Date of Patent: March 29, 2022Assignee: Combinati IncorporatedInventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Publication number: 20220023859Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: ApplicationFiled: February 12, 2021Publication date: January 27, 2022Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Publication number: 20210197202Abstract: The present disclose provides devices, methods and systems that may be used for amplifying and quantifying nucleic acid molecules. Methods for amplifying and quantifying nucleic acids may comprise isolating a sample comprising nucleic acid molecules into a plurality of chambers, performing a polymerase chain reaction on the plurality of chambers, and analyzing the results of the polymerase chain reaction.Type: ApplicationFiled: September 22, 2020Publication date: July 1, 2021Inventors: Andrew Zayac, Sammy Datwani, Megan Dueck, Ju-Sung Hung
-
Patent number: 10737266Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.Type: GrantFiled: March 13, 2019Date of Patent: August 11, 2020Assignee: EMD Millipore CorporationInventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
-
Publication number: 20200248232Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.Type: ApplicationFiled: October 30, 2019Publication date: August 6, 2020Inventors: Ju-Sung Hung, Megan Dueck, Andrew Zayac
-
Publication number: 20200001291Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: ApplicationFiled: February 7, 2019Publication date: January 2, 2020Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Patent number: 10519494Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.Type: GrantFiled: May 9, 2019Date of Patent: December 31, 2019Assignee: COMBINATI INCORPORATEDInventors: Ju-Sung Hung, Megan Dueck, Andrew Zayac
-
Publication number: 20190264260Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.Type: ApplicationFiled: May 9, 2019Publication date: August 29, 2019Inventors: Andrew Zayac, Ju-Sung Hung, Howard Gregg King, David Cox
-
Publication number: 20190210017Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.Type: ApplicationFiled: March 13, 2019Publication date: July 11, 2019Inventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
-
Patent number: 10272429Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.Type: GrantFiled: December 5, 2017Date of Patent: April 30, 2019Assignee: EMD Millipore CorporationInventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
-
Patent number: 10252266Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: GrantFiled: July 3, 2018Date of Patent: April 9, 2019Assignee: COMBINATI INCORPORATEDInventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Publication number: 20180304254Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: ApplicationFiled: July 3, 2018Publication date: October 25, 2018Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Publication number: 20180093266Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.Type: ApplicationFiled: December 5, 2017Publication date: April 5, 2018Inventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
-
Publication number: 20180078935Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.Type: ApplicationFiled: October 13, 2017Publication date: March 22, 2018Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
-
Patent number: 9861982Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.Type: GrantFiled: January 26, 2016Date of Patent: January 9, 2018Assignee: EMD Millipore CorporationInventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
-
Patent number: 9845499Abstract: A microfluidic device can have a plurality of microchambers connected to a microchannel via siphon apertures. The microfluidic device may be formed from a thermoplastic and capped by a thermoplastic thin film. The microfluidic device may be used for digital polymerase chain reactions by forcing reagent into the microchambers at low pressure via an inlet, forcing any gas trapped in the microfluidic device to outgas through the thin film by applying high pressure to the microfluidic device via inlets and outlets of the microfluidic device, and applying air at low pressure to the inlets in order to digitize the chambers such that the reagent in each chamber is isolated from the reagent in other chambers by an air gap. After digitization, the device may be used to run a digital polymerase chain reaction process.Type: GrantFiled: November 29, 2016Date of Patent: December 19, 2017Assignee: COMBINATI INCORPORATEDInventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck