Abstract: The invention provides for methods for isolating large EVs and detecting palmitoyl proteins in the large EVs, as well as methods for detecting clinically significant prostate cancer based on the presence of palmitoyl proteins in the isolated large EVs in a subject in need thereof. The method further comprises administering cancer therapy to the subject.

Abstract: The presently-disclosed subject matter includes labels for use in the identification of shed ALCAM in s sample from a subject. Further included are methods for characterizing, monitoring, evaluating treatment efficacy, or evaluating the progression of cancer in a subject that comprise providing a biological sample from the subject, determining the presence or amount of shed ALCAM in the biological sample, and then comparing the presence or the amount of the shed ALCAM to a reference. Differences between the presence or amount of shed ALCAM relative to the reference can be used to diagnose, prognosticate, treat, monitor, or otherwise characterize a cancer in a subject. Further provided are kits comprising a reagent and/or antibody for diagnosing, prognosticating, monitoring, or otherwise characterizing a cancer in a subject.

Abstract: The presently-disclosed subject matter includes labels for use in the identification of shed ALCAM in s sample from a subject. Further included are methods for characterizing, monitoring, evaluating treatment efficacy, or evaluating the progression of cancer in a subject that comprise providing a biological sample from the subject, determining the presence or amount of shed ALCAM in the biological sample, and then comparing the presence or the amount of the shed ALCAM to a reference. Differences between the presence or amount of shed ALCAM relative to the reference can be used to diagnose, prognosticate, treat, monitor, or otherwise characterize a cancer in a subject. Further provided are kits comprising a reagent and/or antibody for diagnosing, prognosticating, monitoring, or otherwise characterizing a cancer in a subject.

Abstract: A stage adaptor for imaging a biological specimen is described. The adaptor having a housing; a vented chamber contained within the housing; and a removable lid for covering the vented chamber. A depression is provided on the removable lid for receiving an objective from a microscope. An aperture is also provided at the apex of the depression for viewing inside the vented chamber. Also described is an integrated stage adaptor and imaging system as well as a method for imaging the biological specimen using the stage adaptor.

Abstract: The magnetic reversibly attached template (MRAT) are solid devices made of biocompatible material containing a magnetic material. These devices can be made in any shape using microfabrication, milling, and molding and can be positioned and secured using magnetic forces. The applications of the M RAT encompass the patterning of biological and non-biological materials including cells, nucleic acids, peptides, proteins, aqueous solutions, polymers, rubbers, and other reagents on solid surfaces.