Abstract: The invention provides a device for immobilizing and shipping crystals and for data collection via serial crystallography, the device having a first planar substrate defining a first transversely extending aperture, wherein the first substrate has a first laterally facing surface; a second planar substrate defining a second transversely extending aperture coaxial with the first aperture, wherein the second substrate has a second laterally facing surface; a third planar substrate and a fourth planar substrate positioned between the first and second substrates such that the first planar substrate, the second planar substrate, the third planar substrate and the fourth planar substrate are parallel; and a means for reversibly applying axial pressure to the first and second laterally facing surfaces so as to compress the third and fourth substrates together.

Abstract: Provided is a method of treating a disease caused by a nidovirus that encodes Nsp15, such as a coronavirus, an arterivirus, or a torovirus, in a subject in need thereof comprising administering a therapeutically effective amount of an active agent selected from 3?-uridylic acid, 5?-uridylic acid, citrate, methacycline, meclocycline sulfosalicylate, mitoxantrone, epirubicin hydrochloride, daunorubicin hydrochloride, sorafenib, sunitinib malate, primaquine diphosphate, closantel, isopropyl ester of N4-hydroxycytidine, GpU dinucleotide or derivatives thereof, and tipiracil or N-substituted derivatives thereof. Further provided are a method of inhibiting an Nsp15 endoribonuclease of a nidovirus that encodes Nsp15 and compounds of formulas (I?) and (II?).

Abstract: The invention provides a device for immobilizing and shipping crystals and for data collection via serial crystallography, the device having a first planar substrate defining a first transversely extending aperture, opening, wherein the first substrate has a first laterally facing surface; a second planar substrate defining a second transversely extending aperture coaxial with the first aperture, wherein the second substrate has a second laterally facing surface; a third planar substrate and a fourth planar substrate positioned between the first and second substrate such that the first planar substrate, the second planar substrate, the third planar substrate and the fourth planar substrate are parallel; and a means for reversibly applying axial pressure to the first and second laterally facing surfaces so as to compress the third and fourth substrates together.

Abstract: Methods and apparatus for implementing robust and efficient cryoloop auto-centering for crystal location and alignment to the x-ray beam are provided. Image processing techniques are used for recognition of the small protein crystals. The detected crystal location enables crystal positioning in the x-ray beam, and optimization of the x-ray optics such as beam profile and intensity to the size and shape of the protein crystals.

Abstract: A method and robot-based automation system are provided for cryogenic crystal sample mounting, for example, for use for cryogenic crystal sample mounting in the x-ray crystallography station at an x-ray source. The system includes a robot arm carrying a handset. The handset includes a pair of elongated fingers for sample mounting, and each finger carrying a set of strain gauge arrays for providing force sensing. A slim finger design allows a sample mounting process with no interference with the beam stop, cryostreem and x-ray detectors. The handset can detect the contact force intensity and direction; provide a precise gripping action; and feel the results of the gripping. The finger design incorporates a mechanism to maintain the sample temperature well below the cryogenic safety margin for the crystal viability. A Dewar container is provided with an ice control system and liquid nitrogen flow control. A triangular sample magazine maximizes the Dewar space usage.

Abstract: A vector designed to include two tags wherein the first tag improves protein expression, folding and solubility, and the second tag promotes affinity purification, and two distinct recognition sequences for highly specific proteases. Uses of the vector include in vivo protein production.

Abstract: A method and robot-based automation system are provided for cryogenic crystal sample mounting, for example, for use for cryogenic crystal sample mounting in the x-ray crystallography station at an x-ray source. The system includes a robot arm carrying a handset. The handset includes a pair of elongated fingers for sample mounting, and each finger carrying a set of strain gauge arrays for providing force sensing. A slim finger design allows a sample mounting process with no interference with the beam stop, cryostreem and x-ray detectors. The handset can detect the contact force intensity and direction; provide a precise gripping action; and feel the results of the gripping. The finger design incorporates a mechanism to maintain the sample temperature well below the cryogenic safety margin for the crystal viability. A Dewar container is provided with an ice control system and liquid nitrogen flow control. A triangular sample magazine maximizes the Dewar space usage.

Abstract: The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.

Abstract: The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.