Patents by Inventor Andy Wende
Andy Wende has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10088469Abstract: A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma.Type: GrantFiled: August 29, 2013Date of Patent: October 2, 2018Assignee: QIAGEN GmbHInventors: Andy Wende, Sabine Werner, Ralf Himmelreich
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Patent number: 9994891Abstract: The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample.Type: GrantFiled: October 11, 2012Date of Patent: June 12, 2018Assignee: QIAGEN GMBHInventors: Andy Wende, Rainer Dahlke, Ralf Himmelreich, Thomas Rothmann, Michael Eberhard, Gerd Grosshauser, Markus Jeziorski, Hans Attig, Josef Drexler, Eva Holzer
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Patent number: 9822404Abstract: A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate.Type: GrantFiled: November 7, 2013Date of Patent: November 21, 2017Assignee: Qiagen GmbHInventors: Gerd Grosshauser, Andy Wende, Ralf Himmelreich
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Publication number: 20160024563Abstract: The present invention related to methods for improving probe-based melting curve analysis methods. The improvement comprises the inclusion of a compound (A) which is a water-soluble polyanionic co-polymer comprising maleic acid, preferably poly(acrylic acid-co-maleic acid) (PAMA) in the analytical sample that is subjected to the melting curve analysis.Type: ApplicationFiled: April 4, 2014Publication date: January 28, 2016Inventors: Andy Wende, Ralf Himmelreich
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Publication number: 20150329900Abstract: The invention relates to a method of performing a helicase dependent amplification (HDA) or thermophilic helicase dependent amplification of a template nucleic acid comprising: (i) combining in a reaction mixture the template nucleic acid; a forward and a reverse HDA primer; a helicase; at least one DNA polymerase and deoxynucleotide triphosphates (dNTPs), (ii) wherein the reaction comprises a linear polyethylene glycol with the formula wherein n is between 2 to 50, preferably n is selected from the group comprising 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12. The invention also relates to a kit comprising one or more reagents for performing an HDA reaction, wherein a polyethylene glycol is present in the kit.Type: ApplicationFiled: December 17, 2013Publication date: November 19, 2015Inventors: Andy Wende, Sabine Werner
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Publication number: 20150292000Abstract: A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate.Type: ApplicationFiled: November 7, 2013Publication date: October 15, 2015Applicant: QIAGEN GMBHInventors: Gerd Grosshauser, Andy Wende, Rlaf Himmelreich
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Publication number: 20150204843Abstract: A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma.Type: ApplicationFiled: August 29, 2013Publication date: July 23, 2015Applicant: QIAGEN GmbHInventors: Andy Wende, Sabine Werner, Ralf Himmelreich
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Patent number: 9040243Abstract: The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.Type: GrantFiled: September 22, 2011Date of Patent: May 26, 2015Assignee: QIAGEN GMBHInventors: Andy Wende, Francesca Dipasquale, Sabine Werner, Sascha Strauss
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Patent number: 8852863Abstract: The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.Type: GrantFiled: May 4, 2010Date of Patent: October 7, 2014Assignee: Qiagen GmbHInventors: Thomas Rothmann, Holger Engel, Ralf Himmelreich, Andy Wende, Rainer Dahlke
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Publication number: 20140287955Abstract: The present invention pertains inter alia to a method for analysing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample.Type: ApplicationFiled: October 11, 2012Publication date: September 25, 2014Inventors: Andy Wende, Rainer Dahlke, Ralf Himmelreich, Thomas Rothmann, Michael Eberhard, Gerd Grosshauser, Markus Jeziorski, Hans Attig, Josef Drexler, Eva Holzer
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Publication number: 20130224742Abstract: The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.Type: ApplicationFiled: September 22, 2011Publication date: August 29, 2013Applicant: QIAGEN GMBHInventors: Andy Wende, Francesca Dipasquale, Sabine Werner, Sascha Strauss
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Publication number: 20120171728Abstract: Disclosed is a process for amplifying DNA, a process for preparing and amplifying DNA, a kit of parts for DNA amplification and DNA preparation, and the use thereof, all of which are characterized by the use of tetraethylene glycol. Moreover, the use of tetraethylene glycol in a reaction solution by carrying out a DNA amplification and a reaction solution containing tetraethylene glycol for carrying out a DNA amplification are disclosed.Type: ApplicationFiled: June 15, 2010Publication date: July 5, 2012Applicant: QIAGEN GmbHInventors: Andy Wende, Hans Robert Attig, Ralf Himmelreich