Abstract: A non-toxic, alcohol-free disinfectant and sanitizing composition comprising a non-ionic surfactant system, an alkylated vegetable oil, a sodium-containing base, benzalkonium chloride, citric acid and purified water for use on skin, hands and hard surfaces. The materials from which it is manufactured are all renewable, non-toxic, alcohol-free, nonflammable and the composition is readily biodegradable. The composition does not rely upon alcohol to produce its antimicrobial effects. The invention provides effective alternative to alcohol-based disinfectant and sanitizers and may be particularly useful in personal care and cleaning household products for schools, hospitals, restaurants, hotels and supermarkets. Furthermore, the invention also met the Environmental Protection Agency (EPA) standards and protocols for disinfectants on hard surfaces.