Abstract: The present invention discloses a method for preparation of immunologically inert amniotic membranes using foetal membranes collected from programmed caesareans, under sterile conditions. The immunologically inert amniotic membrane of the invention may be applied to the preparation of a product to be used as a skin substitute in 2nd or 3rd degrees burns, as nerve sleeve to guide regeneration of the peripheral nerves, as cornea graft, in the reconstruction of the bladder and urethras, in the correction of cardiac malformations such as inter-auricular and inter-ventricular communications and in the reconstruction of valvular leaflets.