Abstract: Provided is a method for the expression of a TGF-? in a plant. A chimeric nucleic acid sequence comprising: (1) a first nucleic acid sequence capable of regulating the transcription in a plant cell of (2) a second nucleic acid sequence, encoding a TGF-?, and adapted for expression in the plant cell; and (3) a third nucleic acid sequence encoding a termination region functional in said plant cell is introduced into a plant cell and the plant cell grown to produce TGF-?. The nucleic acid sequence may preferably be adapted for expression in a plant chloroplast. It is preferred that the TGF-? is TGF-?3, whether full length or in the form of an active fragment.

Abstract: The invention provides method for producing a transgenic plant comprising a recombinant plastid genome containing an exogenous gene in the absence of a selectable marker gene introduced with the exogenous gene by using direct repeat sequences, nucleic acid constructs containing direct repeat sequences which may be used in the method and transgenic plants produced by the method.