Abstract: The present disclosure provides methods and compositions for genetically modifying lymphocytes, for example T cells and/or NK cells, in shorter times than previously and/or in whole blood or a component thereof. In some embodiments a lymphodepletion filter assembly is used before or after forming a reaction mixture where lymphocytes are contacted with recombinant retroviral particles in a closed system, to genetically modify the lymphocytes.

Abstract: The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and/or NK cells. The methods can include inhibitory RNA molecule(s) and/or engineered signaling polypeptides that can include a lymphoproliferative element, and/or a chimeric antigen receptor (CAR), for example a microenvironment restricted biologic CAR (MRB-CAR). Additional elements of such engineered signaling polypeptides are provided herein, such as those that drive proliferation and regulatory elements therefor, as well as replication incompetent recombinant retroviral particles and packaging cell lines and methods of making the same. Numerous elements and methods for regulating transduced and/or genetically modified T cells and/or NK cells are provided, such as, for example, those including riboswitches, MRB-CARs, recognition domains, and/or pH-modulating agents.

Abstract: In some embodiments, the present disclosure describes a method that includes at least: receiving, by a processor, audience data identifying candidate audience for a website content; generating a plurality of audience groups based on the audience data; receiving user identifying data of a user; generating personalized online content for the user; tagging the personalized online content with a plurality of dynamic tokens to form trackable personalized online content; transmitting instructions to display the trackable personalized online content on a screen of a computing device; receiving user activity tracking data; training a machine learning algorithm, based on the user activity tracking data, to obtain a trained machine learning content recommendation engine; and utilizing the trained machine learning content recommendation engine to identify updated trackable personalized online content based on a plurality of markups to the trackable personalized online content.

Abstract: A computer system receives a definition of a group that includes an indication of a hierarchal rank of group members, a plurality of content items that correspond to the group, and identifying information indicating at least one group member that corresponds to a content item. A search input by a first user is detected and an indication of a hierarchal rank for the first user is stored. Determining a weight to assign to a search result includes comparing a hierarchal rank of at least one group member that corresponds to the first content item with the hierarchal rank of the first user.

Abstract: The present disclosure provides methods and compositions for genetically modifying lymphocytes, for example T cells and/or NK cells, in shorter times than previously and/or in whole blood or a component thereof. In some embodiments a lymphodepletion filter assembly is used before or after forming a reaction mixture where lymphocytes are contacted with recombinant retroviral particles in a closed system, to genetically modify the lymphocytes.

Abstract: A computer system receives a definition of a group that includes an indication of a hierarchal rank of group members, a plurality of content items that correspond to the group, and identifying information indicating at least one group member that corresponds to a content item. A search input by a first user is detected and an indication of a hierarchal rank for the first user is stored. Determining a weight to assign to a search result includes comparing a hierarchal rank of at least one group member that corresponds to the first content item with the hierarchal rank of the first user.

Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration for in vitro fertilization, as well as administration of molecules, or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.

Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.

Abstract: A queue-based non-volatile memory (NVM) hardware assist card, information handling system, and method are disclosed herein.

Abstract: The present disclosure provides methods and compositions for genetically modifying lymphocytes and related methods that include genetically modifying T cells and/or NK cells. The methods use replication incompetent recombinant retroviral particles that comprise a pseudotyping element on their surface and optionally a membrane-bound T cell activation element, such as an anti-CD3, and encode one or more engineered signaling polypeptides that can include a lymphoproliferative element, and/or a chimeric antigen receptor (CAR). The methods can include contacting PBMCs with replication incompetent recombinant retroviral particles for various exemplary time periods, such as less than 24 hours or in some illustrative embodiments less than 15 minutes. In some aspects, the present disclosure provides methods and compositions for genetically modifying lymphocytes, for example T cells and/or NK cells, in whole blood or a component thereof.

Abstract: An information handling system includes an accelerator that advertises, via a single peripheral component interconnect express endpoint, to a processor a plurality of queues from different drives. The processor writes commands and maps each command to target a particular one of the queues. The accelerator performs a queue level logical separation for the mapped command to be processed by the target queue.

Abstract: A computer system receives data that includes indications of a plurality of agenda items that correspond to the group event. During the group event, the system captures, by a media recording device, media data, converts the captured media data to a searchable version of the media data and searches the searchable version of the media data to determine a first time frame of the media data that corresponds to a first agenda item of the plurality of agenda items. The system displays, in a user interface that is accessible to at least one user associated with the group event, the representation of the media data. A first portion of the representation of the media data that corresponds to the first time frame is visually distinguished from at least a portion of the representation of the media data that does not correspond to the first time frame.

Abstract: A computer system receives a definition of a group that includes an indication of a hierarchal rank of group members, a plurality of content items that correspond to the group, and identifying information indicating at least one group member that corresponds to a content item. A search input by a first user is detected and an indication of a hierarchal rank for the first user is stored. Determining a weight to assign to a search result includes comparing a hierarchal rank of at least one group member that corresponds to the first content item with the hierarchal rank of the first user.

Abstract: A computer system generates an action based on a first task includes receiving a definition of a group, stores the definition, and receives task information for the task that corresponds to the group. The task information includes a milestone corresponding to the task and identifying information for the task. The task information is stored, a milestone status is determined, and a prompt is displayed for generating the action. The prompt includes the identifying information for the task and eligible action participants. The eligible action participants include at least a subset of the group members. An indication of selected eligible action participants is received. Action information corresponding to the action is transmitted to the selected eligible action participants.

Abstract: The invention relates to the discovery of novel soluble neutral active Hyaluronidase Glycoproteins (sHASEGPs), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. The invention further comprises sialated and pegylated forms of a recombinant sHASEGP to enhance stability and serum pharmacokinetics over naturally occurring slaughterhouse enzymes. Further described are suitable formulations of a substantially purified recombinant sHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity.

Abstract: A queue-based non-volatile memory (NVM) hardware assist card, information handling system, and method are disclosed herein.

Abstract: An information handling system includes a processor configured to write a first command stride and a second command stride. Each of the first command stride and the second command stride includes logical commands in a single queue, and each of the logical commands is mapped for processing by a peripheral component interconnect express (PCIe). An accelerator may perform a logical separation of the logical commands based on the mapping of each logical command, wherein the logical commands are processed in parallel by the PCIe drives.

Abstract: A domain name registrar may create a customizable universal purchase path for all customers purchasing a given product. The registrar may create and store modular cross sell components that each has material for at least one product offered for sale by the registrar. Thereafter, the registrar may receive a request for a product from a customer. The registrar may determine a probability the customer will complete the purchase if shown upgrades and/or additional products. If the probability is low (or lower than a predefined threshold) the registrar may complete the purchase before displaying additional upgrades and/or additional products to the customer. This will maximize the conversion rate. Alternatively, if the probability is high (or higher than a predefined threshold) the registrar may create final page markups using the modular cross sell components and collected content and display the final page markups to the customer before completing the purchase with the customer.

Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.

Abstract: A domain name registrar may create a customizable universal purchase path for all customers purchasing a given product. The registrar may create and store modular cross sell components that each has material for at least one product offered for sale by the registrar. Thereafter, the registrar may receive a request for a product from a customer. The registrar may determine a probability the customer will complete the purchase if shown upgrades and/or additional products. If the probability is low (or lower than a predefined threshold) the registrar may complete the purchase before displaying additional upgrades and/or additional products to the customer. This will maximize the conversion rate. Alternatively, if the probability is high (or higher than a predefined threshold) the registrar may create final page markups using the modular cross sell components and collected content and display the final page markups to the customer before completing the purchase with the customer.