Abstract: A method for detection of antibodies in a biological sample. The method includes steps of: immobilizing antigens specific to the antibodies between at least two electrodes; binding the antibodies from the biological sample to the antigens; binding probes linked with an enzyme to the antibodies; exposing the enzyme to a metal substrate; depositing a metal layer based on exposing the enzyme to the metal substrate; measuring an electrical property of the metal layer between a first electrode of the at least two electrodes and a second electrode of the at least two electrodes; and detecting, based on measuring the electrical property of the metal layer, the antibodies in the biological sample.

Abstract: Disclosed are methods and devices for platelet function analysis. An impedance-based micro platelet function test device comprises a pair of micro-fabricated 3D electrodes for measuring an electrical impedance between the pair electrodes during a platelet function test, and a microfluidic chamber enclosing the pair of electrodes. At least one of the electrodes comprises an electrode core coated with a metal coating, the metal coating has a patterned surface structure derived from a patterned surface of the electrode core, and the microfluidic chamber has an inlet for accepting a blood sample comprising platelets for the platelet function test. The measured electrical impedance characterizes platelet aggregation in the blood sample. Such micro platelet function test methods and devices provide a low-cost, micro electrical-mechanical system based testing platform that enable accurate and repeatable platelet function analyses.

Abstract: In accordance with an embodiment of the invention, there is provided a method for: a) high-throughput, multiplexed, affinity-based separation of proteins—especially low abundance proteins—from complex biological mixtures such as serum; and b) high-throughput, multiplexed, affinity-based separation of cells—especially rare cells—from complex biological mixtures such as blood or blood fractions. The separation of proteins or cells is achieved based on differential binding to affinity-capture beads of different sizes and then sorting the protein-bound or cell-bound beads using the concept of centrifugal-induced Dean migration in a spiral microfluidic device. This method enables continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding.

Abstract: In accordance with an embodiment of the invention, there is provided a method for: a) high-throughput, multiplexed, affinity-based separation of proteins—especially low abundance proteins—from complex biological mixtures such as serum; and b) high-throughput, multiplexed, affinity-based separation of cells—especially rare cells—from complex biological mixtures such as blood or blood fractions. The separation of proteins or cells is achieved based on differential binding to affinity-capture beads of different sizes and then sorting the protein-bound or cell-bound beads using the concept of centrifugal-induced Dean migration in a spiral microfluidic device. This method enables continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding.