Abstract: Multiplexed lateral flow assays, related methods, and devices are disclosed which are capable of simultaneously detecting multiple analytes. The assays are preferably immunoassays and can be multiplexed spatially, spectrally, and both spatially and spectrally. Multiplexed assays are disclosed employing quantum dots for applications including the detection of human proteins and the monitoring of microorganisms relevant to water contamination. The multiplexed assays can employ one or more species of Surface Enhanced Raman Scattering nanoparticles, with one or more species having a unique Raman shift spectrum. The invention is widely adaptable to a variety of analytes such as biowarfare agents, human clinical markers, and other substances.

Abstract: The present invention provides for a monitoring system and method to monitor an analyte, including determining the presence of the analyte and analyzing the analyte; for example, cellular chorography and biological threats. The system and method use a fluidics apparatus (e.g., a flow cytometer), a computer, a probe synthesizer and/or an initial set of fluorophore conjugated probes to monitor the analyte. The fluidics apparatus is adapted to determine the presence of the analyte based on the intrinsic fluorescence of the analyte, and/or to determine the binding of the probes to the analyte in the sample. The binding information is analyzed by the computer by comparing the binding results with a database of information regarding the analyte. The information gained allows the probe synthesizer to synthesize new probes for a subsequent iteration of the process, wherein additional information regarding the analyte is gained with every iteration.

Abstract: Multiplexed lateral flow assays, related methods, and devices are disclosed which are capable of simultaneously detecting multiple analytes. The assays are preferably immunoassays and can be multiplexed spatially, spectrally, and both spatially and spectrally. Multiplexed assays are disclosed employing quantum dots for applications including the detection of human proteins and the monitoring of microorganisms relevant to water contamination. The invention is widely adaptable to a variety of analytes such as biowarfare agents, human clinical markers, and other substances.