Abstract: A method for isolating D and L amino acids from a racemic mixture of the formula(DL)--H.sub.2 N--CHX--COOY,and salts thereof, wherein X denotes an amino acid side chain and derivatives thereof and Y denotes a suitable alkyl group. The racemic mixture is contacted with water to form an aqueous solution. This aqueous solution is then contacted with an esterase to thereby obtain a solution comprising the resolved amino acids(L)--H.sub.2 N--CHX--COOH and(D)--H.sub.2 N--CHX--COOY.The N-terminus of the resolved (L) and (D) amino acids present in this solution are protected with an N-protecting group R to thereby obtain a solution comprising(L)--R--NH--CHX--COOH and(D)--R--NH--CHX--COOY.The isolation of (L)--R--NH--CHX--COOH from (D)--R--NH--CHX--COOY is achieved in high yields by an acid-base extraction.