Abstract: The present disclosure is directed to polypeptides, such as polypeptides comprising an alkaline phosphatase and a bone-targeting moiety. Also disclosed are expression vectors, such as lentiviral expression vectors, including a nucleotide sequence encoding a polypeptide. Also disclosed are methods of treating hypophosphatasia or treating, mitigating, or preventing one or more symptoms of hypophosphatasia by administering to a subject in need of treatment thereof the disclosed polypeptides or host cells transduced to express any of the disclosed polypeptides.

Abstract: Disclosed herein is a CAR construct encoding an inhibitor of immunodeficiency virus fusion and human CD4 extracellular and transmembrane domains linked to a human CD3? signaling domain, and methods of making and using thereof to treat, reduce, or inhibit HIV in subjects.