Patents by Inventor Anke Krebber

Anke Krebber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nitrilase biocatalysts
    Patent number: 8614081
    Abstract: The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: July 22, 2010
    Date of Patent: December 24, 2013
    Assignee: Codexis, Inc.
    Inventors: Anke Krebber, Emily Mundorff, Marissa Mock, Spiros Kambourakis
  • Penicillin-G acylases
    Patent number: 8569013
    Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: October 29, 2013
    Assignee: Codexis, Inc.
    Inventors: Behnaz Behrouzian, Anke Krebber, Emily Mundorff
  • Methods of using engineered ketoreductase polypeptides for the stereoselective reduction of acetophenones
    Patent number: 8512973
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: August 20, 2013
    Assignees: Codexis, Inc., Pfizer, Inc.
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Huisman
  • PENICILLIN-G ACYLASES
    Publication number: 20120270282
    Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Application
    Filed: July 6, 2012
    Publication date: October 25, 2012
    Applicant: Codexis, Inc.
    Inventors: Behnaz Behrouzian, Anke Krebber, Emily Mundorff
  • Penicillin-G acylases
    Patent number: 8247192
    Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: August 21, 2012
    Assignee: Codexis, Inc.
    Inventors: Behnaz Behrouzian, Anke Krebber, Emily Mundorff
  • NITRILASE BIOCATALYSTS
    Publication number: 20120142063
    Abstract: The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Application
    Filed: July 22, 2010
    Publication date: June 7, 2012
    Applicant: Codexis ,Inc.
    Inventors: Anke Krebber, Emily Mundoff, Marissa Mock, Spiros Kambourakis
  • gene directing the ratio of B2:B1 avermectins
    Patent number: 8008078
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: April 23, 2008
    Date of Patent: August 30, 2011
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Glucose dehydrogenase polypeptides and related polynucleotides
    Patent number: 7939309
    Abstract: The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
    Type: Grant
    Filed: May 28, 2010
    Date of Patent: May 10, 2011
    Assignee: Codexis, Inc.
    Inventors: S. Christopher Davis, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
  • Polynucleotides and methods of directing the ratio of B2:B1 avermectins in host cells
    Patent number: 7858340
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: June 11, 2003
    Date of Patent: December 28, 2010
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
  • GLUCOSE DEHYDROGENASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES
    Publication number: 20100304459
    Abstract: The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
    Type: Application
    Filed: May 28, 2010
    Publication date: December 2, 2010
    Applicant: CODEXIS, INC.
    Inventors: S. Christopher Davis, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
  • Ketoreductase polypeptides and related polynucleotides
    Patent number: 7833767
    Abstract: The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
    Type: Grant
    Filed: October 8, 2009
    Date of Patent: November 16, 2010
    Assignee: Codexis, Inc.
    Inventors: S. Christopher Davis, Gjalt W. Huisman, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
  • PENICILLIN-G ACYLASES
    Publication number: 20100143968
    Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Application
    Filed: November 9, 2009
    Publication date: June 10, 2010
    Applicant: CODEXIS, INC.
    Inventors: BEHNAZ BEHROUZIAN, ANKE KREBBER, EMILY MUNDORFF
  • KETOREDUCTASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES
    Publication number: 20100028972
    Abstract: The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
    Type: Application
    Filed: October 8, 2009
    Publication date: February 4, 2010
    Applicant: CODEXIS, INC.
    Inventors: S. Christopher Davis, Gjalt W. Huisman, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
  • Ketoreductase Polypeptides for the Reduction of Acetophenones
    Publication number: 20090191605
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Application
    Filed: September 13, 2008
    Publication date: July 30, 2009
    Applicant: CODEXIS, INC.
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Huisman
  • Structure-activity relationships
    Publication number: 20090118130
    Abstract: The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
    Type: Application
    Filed: February 12, 2008
    Publication date: May 7, 2009
    Applicant: Codexis, Inc.
    Inventors: Emily Mundorff, Simon Christopher Davis, Gjalt W. Huisman, Anke Krebber, John H. Grate
  • Streptomyces Avermitilis Gene Directing The Ratio of B2:B1 Avermectins
    Publication number: 20090017508
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: April 23, 2008
    Publication date: January 15, 2009
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • gene directing the ratio of B2:B1 avermectins
    Patent number: 7388085
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: February 10, 2003
    Date of Patent: June 17, 2008
    Assignee: Pfizer Inc
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Ketoreductase polypeptides and related polynucleotides
    Publication number: 20060195947
    Abstract: The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
    Type: Application
    Filed: August 11, 2004
    Publication date: August 31, 2006
    Inventors: S. Davis, Gjalt Huisman, Stephane Jenne, Anke Krebber, Lisa Newman
  • Glucose dehydrogenase polypeptides and related polynucleotides
    Publication number: 20050095619
    Abstract: The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
    Type: Application
    Filed: August 11, 2004
    Publication date: May 5, 2005
    Inventors: S. Davis, Stephane Jenne, Anke Krebber, Lisa Newman
  • Directing the ration of B2:B1 avermectins in Streptomyces avermitilis host cells
    Publication number: 20040009560
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: June 11, 2003
    Publication date: January 15, 2004
    Applicant: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Chen Yan, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard