Abstract: Antibodies to antigen presenting cells may be utilized to interfere with the interaction of the antigen presenting cell and immune cells, including T cells. Peptides may be linked to said antibodies thereby generating an immune response to such peptides. Preferably peptides linked to the antibodies are associated with autoimmunity.

Abstract: Antibodies are produced by inoculating a host animal with dendritic cells in combination with a self peptide of interest. In one embodiment, antibodies are produced by incubating the dendritic cells with a self peptide of interest to form an immunogen, incubating B cells from an animal of a different species with a peptide homologous to the self peptide of interest, and immunizing the animal with the immunogen and loaded B cell.

Abstract: Methods of making and selecting engineered antibodies and/or antibody fragments provide maximized binding affinity for a predetermined target and minimized immunogenicity when such antibodies are administered to a target species. Libraries containing variants of the engineered antibodies are also provided. In particularly useful embodiments, anti-PGDF antibodies and compositions are produced which are useful in the treatment of various cancers.