Abstract: A freshness indicator comprises a support structure that is substantially impermeable to fluids. A test device is engaged with the support structure and includes a pathogen detection composition. The pathogen detection composition provides a prescribed visual indication when in the presence of a prescribed type of pathogens and/or a by-product thereof. A flow limiting structure extends over the test device and has an entire perimeter edge portion thereof attached to the support structure in a manner whereby the flow limiting structure and the support structure jointly form a fluid resistant interface around a space in which the test device resides. The flow limiting structure substantially allows a portion of the prescribed type of pathogen and/or a portion of the by-product thereof to enter the space in which the test device resides and substantially inhibits any portion of the pathogen detection composition from escaping from the space in which the test device resides.