Abstract: A well tracking device includes a rectangular frame including four sidewalls. The sidewalls define sets of opposing grooves, and the well tracking device is configured to receive or fit on top of a multi-well plate. The well tracking device also includes a well coordinate indicator positioned on an upper portion of the rectangular frame, that includes labels associated with columns and/or rows of the multi-well plate. The well tracking device also includes at least one slider that is configured to interface with a set of opposing grooves and slide along a width or length of the rectangular frame. The slider includes a plurality of openings disposed along the elongated shape of the slider.

Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into cartridge that is packed with a porous sorbent between opposing frits. Physical properties of the cartridge along with the use of hydrophilic fits allows efficient desalting of biopolymers using a vacuum to pull the sample through the cartridge.

Abstract: A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I: R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20-, —(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

Abstract: A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I: R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20—, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20—.

Abstract: The present disclosure relates to a kit for sample preparation, the kit including a solid support surface with a polymer coating covering the solid support surface, wherein the polymer coating reduces undesired interactions between the sample and the solid support surface, a buffer comprising arginine and methionine, and a vessel for containing the solid support surface and the buffer.

Abstract: The present disclosure relates to a system for separating a sample including a device and a positive pressure source. The device can include a housing with a proximal end having an interface and a proximal end opening, a distal end opening opposite the proximal end opening, and an interior wall defining an interior of the housing; a bottom frit connected to the interior wall, extending across the interior of the housing, and located proximately to the distal end to minimize sample loss; and a resin disposed within the interior of the housing between the bottom frit and the proximal end. The positive pressure source can connect to the interface of the proximal end to apply positive pressure to the sample. A controller can control the applied positive pressure to the sample via the positive pressure source according to a relationship between the bottom frit, the resin, and the positive pressure.

Abstract: A well tracking device includes a rectangular frame including four sidewalls. The sidewalls define sets of opposing grooves, and the well tracking device is configured to receive or fit on top of a multi-well plate. The well tracking device also includes a well coordinate indicator positioned on an upper portion of the rectangular frame, that includes labels associated with columns and/or rows of the multi-well plate. The well tracking device also includes at least one slider that is configured to interface with a set of opposing grooves and slide along a width or length of the rectangular frame. The slider includes a plurality of openings disposed along the elongated shape of the slider.

Abstract: The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.

Abstract: A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.

Abstract: A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I: R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20—, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20—.