Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3? exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3? exonuclease activity; (d) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein

Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and

Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3? exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3? exonuclease activity; (d) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein

Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and

Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3? exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3? exonuclease activity; (d) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein

Abstract: The invention refers to a method and device for ventilating a device for electron beam irradiation of a web (W), the method comprising the steps of: providing a first chamber (107) comprising a web inlet opening (115) and a web outlet opening (115) and a web outlet opening (121), providing a second chamber (111) extending inside the first chamber (107), the second chamber (111) comprising a a web inlet opening (114), a web outlet opening (112), and an electron exit surface (21) through which electrons are adapted to be emitted into the second chamber (111), passing the web (W) through the second chamber (111), and creating a flow of a gaseous fluid through both the first and second chambers (107, 111) in a direction opposite the direction of travel of the web (W) by supplying said fluid into the web outlet opening (121) of the first chamber (107) and providing at least one outlet (113).

Abstract: The present invention refers to a device for electron beam irradiation of at least a first side of a web, the device comprising a tunnel through which the web is adapted to pass, said tunnel being provided with a web inlet portion, a web outlet portion and a central portion adapted to receive at least a first electron beam emitter provided with an electron exit window through which electrons are adapted to be emitted into the tunnel. The tunnel is being angled at least two locations in each of the inlet portion and the outlet portion in such a way that any X-ray formed during the electron beam irradiation of the web is forced to hit the tunnel wall at least twice before exiting the tunnel.

Abstract: The present invention refers to a device for electron beam irradiation of at least a first side of a web, the device comprising a tunnel through which the web is adapted to pass, said tunnel being provided with a web inlet portion, a web outlet portion and a central portion adapted to receive at least a first electron beam emitter provided with an electron exit window through which electrons are adapted to be emitted into the tunnel. The tunnel is being angled at at least two locations in each of the inlet portion and the outlet portion in such a way that any X-ray formed during the electron beam irradiation of the web is forced to hit the tunnel wall at least twice before exiting the tunnel.

Abstract: The invention refers to a method and device for ventilating a device for electron beam irradiation of a web (W), the method comprising the steps of: providing a first chamber (107) comprising a web inlet opening (115) and a web outlet opening (121), providing a second chamber (111) extending inside the first chamber (107), the second chamber (111) comprising a web inlet opening (114), a web outlet opening (112), and an electron exit surface (21) through which electrons are adapted to be emitted into the second chamber (111), passing the web (W) through the second chamber (111), and creating a flow of a gaseous fluid through both the first and second chambers (107, 111) in a direction opposite the direction of travel of the web (W) by supplying said fluid into the web outlet opening (121) of the first chamber (107) and providing at least one outlet (113).

Abstract: An ATM switching node (20) which implements PNNI protocol has a table (known as the consolidated table) which stores plural records, each record associating a connection request input field with corresponding routing information. The node has table maintenance logic (78) which updates the table to consolidate therein both records initiated by operator input and records developed from PNNI updating information. The PNNI updating information is generated by a PNNI protocol unit (56) which consults a topology database of the node. Provision of the consolidate table obviates consultation of multiple tables.