Abstract: Novel Amadori reaction compounds have the formula R.sub.1 --NH--R.sub.2, wherein R.sub.1 comprises the D-form of a 1-amino-1-deoxy-2-ketose radical derived from a sugar radical selected from the group of glucose, xylose, galactose, rhamnose, fructose, mannose, 6-deoxyglucose, glucosamine and galactosamine, and R.sub.2 comprises the L-form of an aminoacid or peptide radical selected from the group of serine, glycine, proline, histidine, arginine, alanine, aspartic acid, glutamic acid, phenylalanine, treonine, cysteine, cystine, glutamine, asparagine, methionine, tyrosine, hydroxyproline, tryptophane, valine isoleucine, lysine and leucine. Compounds and combinations of compounds having the general formula R.sub.1 '--NH--R.sub.2 ', wherein R.sub.1 ' comprises a 1-amino-1-deoxy-2-ketose radical derived from the group of simple sugars, oligo- and polysaccharides, and R.sub.

Abstract: The immunological activity of certain bioactive substances is determined by treating a human peripheral blood leukocyte (PBL) culture or a suspension of BALB/c mice resident peritoneal cells (RPC), with a solution of the substance to be tested in order to induce production of cytokines which then are determined according to standard identification methods; amplification of the results may be achieved by admixing, to the solution to be tested, a non-steroidal anti-inflammatory drug, preferably indomethacin. The method is also useful for determining the immunological response of a human individual to a therapy using a cytokine inducing substance.