Abstract: The present disclosure provides materials and methods related to gene stacking. In particular, the present disclosure provides gene stacking systems and components thereof and methods for delivering target DNA inserts to acceptor vectors and target cells such as plant cells. The provided gene stacking systems and components thereof enable an increased DNA cargo size limit, such as an acceptor vector configured to contain up to 300 kb of target DNA inserts. The provided gene stacking systems and components thereof also enable editing of constructs post-assembly. Additionally, the provided gene stacking systems are compatible with all major Type IIS cloning technologies in plants.

Abstract: The invention relates to logic-gate-based Type II or Type V CRISPR-Cas constructs and methods for modifying gene expression using the CRISPR-Cas constructs and CRISPR-Cas effector proteins.

Abstract: The field of the invention relates to plants and plant genes, including both plant mutants and transgenic plants containing a gene that confers an ethylene insensitive phenotype. Also encompassed by the invention are methods of using the disclosed plant gene to confer an ethylene insensitive phenotype.