Abstract: The present invention is directed to a novel multi-spectral exogenous fluorescence polarization imaging technique that enables rapid imaging of large tissue fields. The imaging device includes a tunable monochromatic light source and a CCD camera. Linear polarizers are placed into both the incident and collected light pathways in order to obtain fluorescence polarization or/and anisotropy image. To acquire exogenous fluorescence image, fluorescent contrast agents are delivered to a target tissue.

Abstract: A device for wide-field and high resolution imaging of an object surface includes first and second imaging modalities, a lens associated with the second imaging modality. The first imaging modality has a high resolution imaging means with a first observation line. The second imaging modality is arranged in an image plane at a first angle with respect to an object plane and has a second observation line and a wider imaging field than the first imaging modality. The lens associated with the second imaging modality is arranged in a lens plane at a second angle with respect to the object plane, where the second angle being equal to about one-half of the first angle. The first and second imaging modalities are mutually arranged such that the first and second optical axes intersect at a point on the object plane.

Abstract: The present invention is directed to a novel multi-spectral exogenous fluorescence polarization imaging technique that enables rapid imaging of large tissue fields. The imaging device includes a tunable monochromatic light source and a CCD camera. Linear polarizers are placed into both the incident and collected light pathways in order to obtain fluorescence polarization or/and anisotropy image. To acquire exogenous fluorescence image, fluorescent contrast agents are delivered to a target tissue.