Abstract: An aspect of the invention relates to a method for the diagnosis and/or prognosis of drug resistance, wherein single cell chromatin states are profiled in cells obtained from a subject by using a microfluidic system.

Abstract: An aspect of the invention relates to a method for the diagnosis and/or prognosis of drug resistance, wherein single cell chromatin states are profiled in cells obtained from a subject by using a microfluidic system, the method comprising the steps of: a. providing at least a droplet of first type, wherein said droplet of first type comprises i. a biological element, ii. a lysis buffer, and iii. a nuclease, b. collecting said droplet of first type under conditions that temporarily inactivate said nuclease, c. incubating said droplet of first type, thereby reactivating said nuclease, d. providing at least a droplet of second type, wherein said droplet of second type comprises a nucleic acid sequence, e. merging said droplets of first and second type, thereby generating a droplet of third type, f. incubating said droplet of third type, thereby linking said nucleic acid sequence to one or more genomic region(s) of interest, g. sequencing said one or more genomic region(s) of interest.

Abstract: A first aspect of the present invention is directed to a method for the detection of a compound of interest in a microfluidic system. A second aspect of the present invention relates to the use of the method according to the first aspect for monitoring a biological event. A further aspect of the present invention is directed to a microfluidic system and the use thereof for carrying out the method according to the first aspect.

Abstract: The present invention concerns a method for capturing and barcoding nucleic acid from single cells, a plurality of microfluidic droplets and a method for preparing said plurality of microfluidic droplets.