Patents by Inventor Annalisa Di Ruscio

Annalisa Di Ruscio has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNMT1-Specific Aptamers and Production and Uses Thereof
    Publication number: 20220411799
    Abstract: An aptamer, capable of inhibiting DNA methyltransferase 1 (DNMT1) for use in therapy of diseases characterised by aberrant DNA methylation, e.g. cancer. Method for identifying inhibitors of DNA methyltransferase. An aptamer, capable of inhibiting DNA methyltransferase 1 (DNMT1) for use in therapy of diseases characterised by aberrant DNA methylation, e.g. cancer. SELEX method for identifying aptamers of DNA methyltransferase optionally using 2-fluoro-pyrimindine nucleotide derivatives.
    Type: Application
    Filed: October 28, 2020
    Publication date: December 29, 2022
    Inventors: Daniel Geoffrey Tenen, Carla Lucia Esposito, Annalisa Di Ruscio, Vittorio De Franciscis, Alexander K. Ebralidze
  • METHODS AND COMPOSITIONS FOR GENE SPECIFIC DEMETHYLATION AND ACTIVATION
    Publication number: 20220290139
    Abstract: Provided herein are methods and agents for gene specific demethylation and/or activation. Oligonucleotide constructs are provided, the oligonucleotide constructs including: [1] a targeting portion having sequence complementarity and binding affinity with a region of genomic DNA within a gene, near a gene, or both; and [2] a single guide RNA (sgRNA) scaffold portion, wherein a tetra-loop portion of the sgRNA is modified and includes an R2 stem loop of DNMT1-interacting RNA (DiR), and wherein a stem loop 2 portion of the sgRNA is modified and includes an R5 step loop of DiR. The oligonucleotide constructs may be used, together with deactivated (dead) Cas9 (dCas9) for providing gene specific demethylation and/or activation of gene(s) of interest in a cell or subject in need thereof.
    Type: Application
    Filed: July 15, 2020
    Publication date: September 15, 2022
    Inventors: Yanjing LIU, Daniel G. TENEN, Annalisa DI RUSCIO, Alexander K. EBRALIDZE
  • Methods and compositions for gene-specific demethylation by DNA methyltransferase (DNMT)-RNA interaction
    Patent number: 10941399
    Abstract: The present invention relates to chimeric RNA oligonucleotides that are single-stranded oligonucleotides. These compounds are capable of targeting particular genes and reducing DNA methyltransferase activity. Accordingly, these compounds are particularly useful in the treatment of disease associated with aberrant DNA methyltransferase activity, such as cancer or a genetic disorder.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: March 9, 2021
    Assignees: Beth Israel Deaconess Medical Center, Inc., Università Cattolica del Sacro Cuore
    Inventors: Annalisa Di Ruscio, Alexander K. Ebralidze, Daniel G. Tenen, Giuseppe Leone
  • CHIMERIC RNA OLIGONUCLEOTIDES AND USES THEREOF
    Publication number: 20200063130
    Abstract: The present invention relates to chimeric RNA oligonucleotides that are single-stranded oligonucleotides. These compounds are capable of targeting particular genes and reducing DNA methyltransferase activity. Accordingly, these compounds are particularly useful in the treatment of disease associated with aberrant DNA methyltransferase activity, such as cancer or a genetic disorder.
    Type: Application
    Filed: July 1, 2019
    Publication date: February 27, 2020
    Inventors: Annalisa DI RUSCIO, Alexander K. EBRALIDZE, Daniel G. TENEN, Giuseppe LEONE
  • CHIMERIC RNA OLIGONUCLEOTIDES AND USES THEREOF
    Publication number: 20140171492
    Abstract: The present invention relates to chimeric RNA oligonucleotides that are single-stranded oligonucleotides. These compounds are capable of targeting particular genes and reducing DNA methyltransferase activity. Accordingly, these compounds are particularly useful in the treatment of disease associated with aberrant DNA methyltransferase activity, such as cancer or a genetic disorder.
    Type: Application
    Filed: April 13, 2012
    Publication date: June 19, 2014
    Applicants: Universita Cattolica del Sacro Cuore, Beth Israel Deaconess Medical Center, Inc.
    Inventors: Annalisa Di Ruscio, Alexander K. Ebralidze, Daniel G. Tenen, Giuseppe Leone