Abstract: The present disclosure provides a process for production of glucose having purity greater than 98% from pretreated cellulosic biomass comprising 50-95% cellulose, using thermo-chemical and enzymatic treatments. The process of the disclosure involves mild acid and/or alkali treatment of the pretreated cellulosic biomass for production of treated cellulosic biomass residue comprising polysaccharides containing greater than 98% glucose. The treatment results in high purity cellulose that is highly amenable to enzymatic hydrolysis and yields glucose having purity greater than 98%. The process of the disclosure also involves membrane separation of enzymes and oligosaccharides for recycling and separation of glucose to avoid product feedback inhibition.

Abstract: The present disclosure provides a process for production of glucose having purity greater than 98% from pretreated cellulosic biomass comprising 50-95% cellulose, using thermo-chemical and enzymatic treatments. The process of the disclosure involves mild acid and/or alkali treatment of the pretreated cellulosic biomass for production of treated cellulosic biomass residue comprising polysaccharides containing greater than 98% glucose. The treatment results in high purity cellulose that is highly amenable to enzymatic hydrolysis and yields glucose having purity greater than 98%. The process of the disclosure also involves membrane separation of enzymes and oligosaccharides for recycling and separation of glucose to avoid product feedback inhibition.

Abstract: The present invention provides a process for enzyme mediated hydrolysis of biomass for production of soluble sugars, wherein the said process comprises of steady addition of small portions of biomass to enzyme solution, enabling rapid solubilization of biomass. The process used for enzymatic saccharification allows for increased biomass loading, enzyme recycle and mitigation of substrate and product inhibitory effect. The recycling of unhydrolysed biomass along with soluble enzyme ensures complete reuse of the said enzyme for effective repeated hydrolysis thereby increasing the overall productivity of enzyme used.

Abstract: An efficient process for enzymatic hydrolysis of fats and oils in a homogenous mixture is provided herein. The present invention in particular provides a process for production of fatty acids, sn-regio mono-acylglycerol (MAG), sn-regio di-acyl-glycerols (DAG), and glycerol from fats, wherein more than 98% fats can be converted into the desired product. The present invention also provides a process for the production of fatty acids and glycerol, virtually free of sn-regio diacyl-glycerols (DAG) and comprising less than 5% sn-regio mono-acylglycerol (MAG) in the end product.

Abstract: The present invention provides a process for enzyme mediated hydrolysis of biomass for production of soluble sugars, wherein the said process comprises of steady addition of small portions of biomass to enzyme solution, enabling rapid solubilization of biomass. The process used for enzymatic saccharification allows for increased biomass loading, enzyme recycle and mitigation of substrate and product inhibitory effect. The recycling of unhydrolysed biomass along with soluble enzyme ensures complete reuse of the said enzyme for effective repeated hydrolysis thereby increasing the overall productivity of enzyme used.

Abstract: An efficient process for enzymatic hydrolysis of fats and oils in a homogenous mixture is provided herein. The present invention in particular provides a process for production of fatty acids, sn-regio mono-acylglycerol (MAG), sn-regio di-acyl-glycerols (DAG), and glycerol from fats, wherein more than 98% fats can be converted into the desired product. The present invention also provides a process for the production of fatty acids and glycerol, virtually free of sn-regio diacyl-glycerols (DAG) and comprising less than 5% sn-regio mono-acylglycerol (MAG) in the end product.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.