Abstract: A computer-implemented method for determining a reference proteomic profile of a pathological condition in a subject, comprising steps of: label free quantification of the relative protein abundances of a tissue sample from at least one subject for which a diagnosis of a pathological condition of said tissue has been previously established, said sample containing non-pathological and pathological tissue, determining the proteomic profile of said sample by calculating the ratio of the relative abundances, for each protein, of said protein in the pathological tissue with respect to said protein in the non-pathological tissue, determining a reference proteomic profile for the previously diagnosed pathological condition, by means of a statistical test established between at least two groups of subjects.

Abstract: Some embodiments are directed to a method for the subcellular proteomic analysis of a test biological sample, including metabolic isotopic labelling of proteins of a test biological sample, fixing of the sample, labelling of the test subcellular compartment, laser microdissection of said subcellular compartment, extracting the proteins of said subcellular compartment, reversion of the fixing and proteolysis, analyzing the peptides obtained by mass spectrometry, and identifying the analyzed peptides.