Patents by Inventor Anne Breuner

Anne Breuner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Stable genomic integration of multiple polynucleotide copies
    Patent number: 11174487
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: November 16, 2021
    Assignee: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Stable Genomic Integration of Multiple Polynucleotide Copies
    Publication number: 20170218381
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: April 7, 2017
    Publication date: August 3, 2017
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Methods for producing secreted polypeptides
    Patent number: 9428558
    Abstract: The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: August 30, 2016
    Assignee: Novozymes A/S
    Inventors: Steen Troels Joergensen, Anne Breüner
  • Stable Genomic Integration of Multiple Polynucleotide Copies
    Publication number: 20140273237
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: March 20, 2014
    Publication date: September 18, 2014
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Methods for Producing Secreted Polypeptides
    Publication number: 20140073009
    Abstract: The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.
    Type: Application
    Filed: March 22, 2012
    Publication date: March 13, 2014
    Applicant: Novozymes A/S
    Inventors: Steen Troels Joergensen, Anne Breüner
  • Stable Genomic Integration of Multiple Polynucleotide Copies
    Publication number: 20080085535
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for Promoter carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: October 19, 2005
    Publication date: April 10, 2008
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen